诱导型一氧化氮合酶在前列腺癌中的表达及与肿瘤血管生成的关系

目的]通过测定诱导型一氧化氮合酶(iNOS)在前列腺增生症(BPH)、前列腺癌(PC)中表达情况以及微血管密度(MVD)计数,探讨其与肿瘤血管生成的关系.方法]采用链霉菌素抗生物蛋白-过氧化酶方法,分别用iNOS多克隆抗体和CD34单克隆抗体对30例BPH、50例PC行免疫组织化学染色,采用免疫组织化学染色评分方法...

Expression of Inducible Nitric Oxide Synthase in Prostate Cancer and It Relationship with Tumor Angiogenesis

Objective]To detect the expression of inducible nitric oxide synthase （iNOS） and microvessel density（MVD） count in benign prostatic hyperplasia （BPH） and prostate cancer（PC） in order to explore their relationship with tumor angiogenesis. Methods] Polyclonal antibody against iNOS and CD34-antibody were used for immunohistochemieal staining of 30 cases with BPH and 50 cases with PC by streptin anti-bioprotein peroxidase assay. mmunohistochemical staining score was used for the qualitative score of iNOS-positive sections. Weinder method was used for the CD34-1abelled MVD count. Results]The expression of iNOS in BPH was low and in PC was high, and there was significant difference（ P d0.05）. There was no significant difference in the expression of iNOS among the PC with various pathological grades and clinical stages（ P 〉0. 05）. According to cytopathology, the PC group and BPH group were divided into high differentiated, moderate differentiated and low-differentiated group, respectively. According to clinical stage, the PC group and BPH group were divided into T1 ,T2 ,T3 and T4 group, respectively. By statistically analysis, there were significant differences in MVD count among groups（ P 〈0. 01）. Conclusion] iNOS is associated with the genesis ancl development of prostate cancer. The mechanism is that iNOS may promote the tumor angiogenesis.