| 昆明山海棠总生物碱对LPS诱导的小鼠RAW264.7细胞分泌TNF-αNO的影响 |
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| 引用本文： | 姜晓,敖林,崔志鸿,黄晓春,曹佳,刘晋祎.昆明山海棠总生物碱对LPS诱导的小鼠RAW264.7细胞分泌TNF-αNO的影响[J].中医药导报,2011,17(3):82-84. |
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| 作者姓名： | 姜晓 敖林 崔志鸿 黄晓春 曹佳 刘晋祎 |
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| 作者单位： | 第三军医大学军事预防医学院军事毒理学教研室,重庆,400038 |
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| 基金项目： | 重庆市自然科学基金(CSTC2009BB5026) |
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| 摘 要： | 目的:研究THHta对LPS诱导的小鼠RAW264.7细胞分泌TNF-α及NO的影响,探讨THHta的抗炎作用机理.方法:CCK8法测定THHta对RAW264.7细胞增殖的影响;LPS刺激RAW264.7细胞建立体外炎症模型;ELISA法检测THHta对LPS诱导的RAW264.7细胞分泌TNF-α的影响;硝酸还原酶...
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| 关 键 词： | 昆明山海棠 总生物碱 脂多糖 TNF-α NO |
Effects of Total alkaloids of Tripterygium Hypoglaucum (Levi) Hutch on the production of TNF-a and NO by LPS-induced RAW264.7 cells |
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| JIANG Xiao,AO Lin,CUI Zhi-hong,HUANG Xiao-chun,CAO Jia,LIU Jin-yi.Effects of Total alkaloids of Tripterygium Hypoglaucum (Levi) Hutch on the production of TNF-a and NO by LPS-induced RAW264.7 cells[J].GUIDING JOURNAL OF TCM,2011,17(3):82-84. |
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| Authors: | JIANG Xiao AO Lin CUI Zhi-hong HUANG Xiao-chun CAO Jia LIU Jin-yi |
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| Institution: | (Department of Military Toxicology,College of Preventive Medicine,Third Military Medical University, Chongqing 400038,China) |
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| Abstract: | Objective To investigate the anti-inflammatory mechanism of THHta on LPS-induced RAW264.7 cells.Methods The cytotoxicity of THHta on RAW264.7 cells was detected by CCK8 method.Inflammatory cellular model was established by lipopolysaccharide(LPS) interfering on RAW264.7 cell line.Levels of TNF-α and NO in supernatant were assayed by ELISA or Nitrate acid reductase method.Result THHta inhibited the secretion of TNF-α and NO in LPS-induced RAW264.7 cells.Conclusion The anti-inflammatory effects of THHta may be mediated in part by inhibiting the production of inflammatory mediators. |
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| Keywords: | NO |
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