慢性肝病,肝癌及癌旁组织的免疫组织化学和组织化学研究

作者应用PAP组织化学法和组织化学染色法,对39例慢性肝病(CLD)、8例肝癌(HCC)和癌旁组织HBsAg、HBcAg表达和分布与CLD病变活动性的关系,以及乙肝病毒(HBV)与HCC发生的关系进行了探讨,并观察了CLD中网状纤维和胶原纤维的改变。发现慢活肝和活动性肝硬变以浆膜HBcAg和胞膜HBsAg表达为主,与活动性病变关系密切,观察到网状纤维较早出现于碎屑状坏死区。同时发现癌旁肝组织HBsAg和HBcAg检出数较高,且常见于双核肝细胞内,而肝癌为阴性。结果提示:浆膜HBcAg和胞膜HBsAg可能为CLD肝细胞免疫损伤的主要靶抗原,是引起CLD病变反复活动、呈慢性化的主要原因之一。结果也提示:HBV感染与HCC发生的关系极为密切。

IMMUNOHISTOCHEMICAL AND HISTOCHEMICAL STUDIES OF CHRONIC LIVER DISEASES, HEPATOCELLULAR CARCINOMA AND PERICANCEROUS LIVER TISSUES

Thirty-nine cases of chronic liver diseases(CLD), 8 cases of hepatocellular carcinoma(HCC) and pericancerous liver tissues were studied by PAP method. We found that the main expression patterns of cytoplasmic membranous HBcAg and membranous HBsAg of active CLD were associated with active foci. Reticular fiber was found in early piecemeal necrotic areas. Meanwhile, a high detection of nuclear HBcAg and cytoplasm HBsAg was obtained in pericancerous liver tissues and no detection of HBcAg or HBcAg was obtained in HCC. HBcAg and HBsAg were also found in double nuclear hypatocytes of pericancerous liver tissues. The present results suggest that cytoplasm membranous HBcAg and membranous HBsAg may be the main target antigens causing repeatedly focal necrosis of hepatocytes and is one of the main causes of chronicity of CLD. The results also suggest that HBV is closely associated with HCC.