| 应用噬菌体7肽库筛选泡球蚴Em18抗原模拟表位的初步研究 |
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| 引用本文： | 张蓓,杨晨晨,林仁勇,王俊芳,张春桃,王俨,卢晓梅,张静萍,张琰,许晏,温浩.应用噬菌体7肽库筛选泡球蚴Em18抗原模拟表位的初步研究[J].新疆医科大学学报,2007,30(4):343-345. |
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| 作者姓名： | 张蓓 杨晨晨 林仁勇 王俊芳 张春桃 王俨 卢晓梅 张静萍 张琰 许晏 温浩 |
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| 作者单位： | 1. 新疆医科大学第一附属医院,新疆包虫病基础医学重点实验室,新疆,乌鲁木齐,830054
2. 新疆医科大学基础医学院免疫学教研室,新疆,乌鲁木齐,830054 |
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| 基金项目： | 国家自然科学基金;新疆维吾尔自治区高校科研计划创新研究群体基金;新疆重点实验室基金 |
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| 摘 要： | 目的：从噬菌体7肽库中筛选泡球蚴Em18抗原模拟表位,为研究和开发新的包虫病诊断抗原提供实验依据.方法：用纯化后的rEm18-GST免疫新西兰白兔,获得抗rEm18-GST的多克隆抗体,进一步纯化,获得抗Em18多克隆抗体IgG,以之作为靶分子,免疫筛选噬菌体随机7肽库.经过5轮的淘筛过程,随机挑取9个蓝色噬菌斑扩增,核苷酸序列测定分析并与Em18进行同源性比较.结果：经5轮筛选后,阳性克隆得到富集,9个噬菌体克隆的氨基酸序列与Em18无同源性.结论：所得肽段可能是Em18抗原模拟表位.
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| 关 键 词： | 噬菌体肽库 模拟表位 |
| 文章编号： | 1009-5551（2007）04-0343-03 |
| 修稿时间： | 2006-05-11 |
Original research of Echinococcus multilocularis diagnostic antigen Em18 with phage 7-mer Peptide Library |
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| ZHANG Bei, YANG Chen-chen, LIN Ren-yong, et al.Original research of Echinococcus multilocularis diagnostic antigen Em18 with phage 7-mer Peptide Library[J].Journal of Xinjiang Medical University,2007,30(4):343-345. |
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| Authors: | ZHANG Bei YANG Chen-chen LIN Ren-yong |
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| Institution: | Xinjiang Key Laboratory of Hydatid Fundamental Medical Research, First Affililated Hospital, Xinjiang Medical University, Urumqi 830054, China |
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| Abstract: | Objective: To screen special mimic epitopes of Echinococcus multilocularis antigen Em18 from 7 peptide library for researching and exploring new diagnostic antigens of Echinococcus multilocularis. Methods: The New-Zealand White Rabbits were immunized with the purified recombinant rEm18-GST . After obtaining the purified polyclonal antibody against rEm18-GST, we use the specific anti-Em18 IgG to screen the phage 7-mer peptide library. After 5 rounds panning, 9 clones were picked out randomly to proliferate. The proliferated clones were sequenced and their amino acid sequences were compared with Em18. Results: After 5 rounds selected and the amino acid sequences of 9 clones showed no homology with Em18. Conclusion: The peptide may be the mimic epitopes of antigen Em18. |
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| Keywords: | Em18 |
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