灯盏花素对左心缺血再灌注致肺损伤家兔膈神经放电的影响

目的探讨灯盏花素对左心缺血再灌注致肺损伤的保护作用。方法 30只新西兰家兔随机分为假手术组、模型组和治疗组,每组10只,制备左心缺血再灌注肺损伤模型,并与BL-420生物信号采集分析系统相连;假手术组不做左冠状动脉的前降支结扎,其余同模型组。治疗组缺血10 min后静脉给予灯盏花素10 mg.kg-1。记录缺血30 min,再灌注20、40 min 3个时间点3组家兔膈神经放电曲线,测定放电幅度、放电持续时间和放电频率。结果与假手术组比较,模型组家兔各时间点膈神经放电幅度显著减小,放电持续时间缩短,放电频率增加,其差异均有统计学意义(P<0.05);与模型组比较,治疗组膈神经放电幅度显著增加,放电持续时间延长,膈神经放电频率降低,其差异亦均有统计学意义(P<0.05)。结论灯盏花素可改善左心缺血再灌注急性肺损伤家兔膈神经放电曲线参数,灯盏花素对左心缺血再灌注后的呼吸功能具有保护作用。

Effect of breviscapine on phrenic nerve discharge curve on acute lung injury induced by left heart ischemic reperfusion of rabbits

Objective To investigate the protective effects of breviscapine on acute lung injury induced by left heart ischemic reperfusion of rabbits.Methods A total of 30 rabbits were randomly divided into sham operation group,model group and treatment group,ten rabbits in each group.The model of left heart ischemia-reperfusion was established through ligaturing and loosing the anterior descending branch.The model group were only copied the models,the treatment group were not only copied the modle but also cured by breviscapine injection(10 mg·kg-1) 10 minutes after ischemia,the sham operation group didn′t ligature the anterior descending branch.The discharge of phrenic nerve were recorded with BL-420 computer system 30 minutes after ligaturing,20 minutes after loosing and 40 minutes after loosing,respectively.The amplitude,continual time and frequency of the phrenic nerve discharges were measured.Results Compared with sham operation group,the amplitude and continual time of phrenic nerve discharge were significantly lower and the discharge frequency of phrenic nerve increased significantly in model group at the 3 time points(P<0.05).Compared with model group,the amplitude and continual time of phrenic nerve discharge increased significantly and the discharge frequency of phrenic nerve decreased significantly in treatment group at the 3 time points(P<0.05).Conclusion Breviscapine can improve discharge curve parameter of phrenic nerve and has protective effect on acute lung injury induced by left heart ischemic reperfusion model of rabbits.