| BCG HSP65-人HER-2/neu T细胞表位融合蛋白基因的扩增及序列分析 |
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| 引用本文： | 王丽娟,吴士彬,于永利,王丽颖. BCG HSP65-人HER-2/neu T细胞表位融合蛋白基因的扩增及序列分析[J]. 蚌埠医学院学报, 2002, 27(4): 292-294. DOI: 10.3969/j.issn.1000-2200.2002.04.005 |
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| 作者姓名： | 王丽娟 吴士彬 于永利 王丽颖 |
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| 作者单位： | 1. 蚌埠医学院,病原生物学教研室,安徽,蚌埠,233003;2. 安徽省蚌埠市第三人民医院,泌尿外科,233003;3. 吉林大学基础医学院免疫学教研室,吉林,长春,130021;4. 吉林大学基础医学院分子生物学教研室,吉林,长春,130021 |
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| 摘 要： | 目的:获得卡介苗热休克蛋白65(BCG HSP65)与人HER-2/neu T细胞表位(GP2)构成的融合蛋白(BCG HSP65-GP2)基因,并对其进行测序.方法:从卡介苗结核杆菌中提取其基因组DNA,设计一对寡核苷酸引物,采用聚合酶链反应(PCR)获得BCG HSP65基因,再设计一对寡核苷酸引物,再次PCR,获得BCG HSP65-GP2基因,DNA序列分析BCG HSP65-GP2基因.结果:本实验采用两轮PCR方法获得的基因片段长度为1 700 bp,DNA序列分析确证为编码BCG HSP65-GP2融合蛋白的序列. 结论:采用PCR获得BCG HSP65-GP2基因序列是正确的,为进一步基因克隆和表达奠定基础.
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| 关 键 词： | 基因表达调节 卡介苗热休克蛋白65 DNA |
| 文章编号： | 1000-2200(2002)04-0292-03 |
| 修稿时间： | 2002-01-04 |
Amplification and sequence analysis of fusion protein gene of BCG-HSP65 and human HER-2/neu T cell epitope |
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| WANG Li juan,WU Shi bin,YU Yong li,WANG Li ying. Amplification and sequence analysis of fusion protein gene of BCG-HSP65 and human HER-2/neu T cell epitope[J]. Journal of Bengbu Medical College, 2002, 27(4): 292-294. DOI: 10.3969/j.issn.1000-2200.2002.04.005 |
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| Authors: | WANG Li juan WU Shi bin YU Yong li WANG Li ying |
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| Abstract: | Objective:To obtain the sequence of fusion protein gene of BCG HSP65 and human HER 2/neu T cell epitope.Methods:BCG HSP65 GP 2 gene was obtained by using two round PCR and then sequenced.Results:The length of the reconstructed gene is 1 700 bp. Sequencing analysis confirmed that it was the right sequence encoding fusion protein of BCG HSP65 GP 2. Conclusions:The sequence of BCG HSP65 GP 2 obtained by two round PCR is corrected.It has laid the foundation for the further researching work on the cloning and expression of BCG HSP65 GP 2. |
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| Keywords: | gene expression regulation BCG vaccine heat shock protein 65 DNA |
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