| CCR7基因修饰未成熟树突状细胞诱导小鼠皮肤移植免疫耐受的研究 |
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| 引用本文： | 辛海明,彭毅志,郭浩,袁志强,童亚林.CCR7基因修饰未成熟树突状细胞诱导小鼠皮肤移植免疫耐受的研究[J].重庆医学,2009,38(19). |
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| 作者姓名： | 辛海明 彭毅志 郭浩 袁志强 童亚林 |
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| 作者单位： | 1. 解放军第一八一医院烧伤整形外科,桂林,541002
2. 第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆,400038 |
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| 基金项目： | 国家自然科学基金资助项目，全军"十一五"计划课题项目 |
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| 摘 要： | 目的 研究CCR7基因重组腺病毒体外转染未成熟树突状细胞(imDCs)对小鼠同种异体皮肤移植免疫耐受的影响.方法 小鼠骨髓细胞经梯度离心及rmIL-4、rmGM-CSF诱导培养未成熟树突状细胞;CCR7重组腺病毒经增强离心法转染未成熟树突状细胞;体外观察转染细胞混合淋巴细胞反应;取CCR7修饰imDCs于皮肤移植前2d、移植后第5天,经腹部注入受体小鼠,观察皮肤移植物存活时间和病理变化.结果 CCR7基因成功转染入imDCs;腺病毒转染后imDCs刺激T细胞增殖的能力较imDCs组增强但弱于mDCs组,IL-10可以明显降低刺激能力;CCR7腺病毒转染组皮肤存活时间长于转染前,IL-10能显著延长皮肤存活时间.结论 CCR7重组腺病毒转染imDCs后,imDCs刺激T细胞增殖的能力部分增强,皮肤移植物存活时间明显延长.
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| 关 键 词： | 未成熟树突状细胞 转染 免疫耐受 |
Research on induction of immune tolerance to murine skin allograft by CCR7 gene modified immature dendritic cells |
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| XIN Hai-ming ,PENG Yi-zhi ,GUO Hao,et al..Research on induction of immune tolerance to murine skin allograft by CCR7 gene modified immature dendritic cells[J].Chongqing Medical Journal,2009,38(19). |
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| Authors: | XIN Hai-ming PENG Yi-zhi GUO Hao |
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| Institution: | XIN Hai-ming 1,PENG Yi-zhi 1,GUO Hao1,et al.(1.State Key Laboratory of Trauma,Burns , Combined Injury,Institute of Burns,Southwest Hospital,Third Military Medical University,Chongqing 400038,China,2.Department of Burns , Plastic Surgery,No.181th Hospital of PLA,Guilin 541002,China) |
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| Abstract: | Objective To investigate the influence of immune tolerance to murine skin allograft transfected with CCR7 recombinant adenovirus in vitro.Methods Immature dendritic cells generated in the presence of recombinant murine interleukin-4 and granulocyte/macrophage colony-stimulating factor from murine bone marrow by gradient centrifugation method;CCR7 recombinant adenovirus vector was transfected to immature dendritic cells by centrifugal force method;The mixed lymphocyte reaction of imDCs were observed after tr... |
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| Keywords: | CCR7 MST |
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