小细胞肺癌组织中p53基因改变的研究

为探讨小细胞肺癌（ＳＣＬＣ）发生的分子机制，应用免疫组化和聚合酶链反应—单链构象多态性分析的方法，对１４例ＳＣＬＣ组织进行ｐ５３基因突变研究。结果显示，有９例出现ｐ５３蛋白阳性，阳性率为６４．３％（９／１４）。ｐ５３基因第５，６，７，８外显子突变率分别为２１．４％（３／１４），１４．３％（２／１４），１４．３％（２／１４），７．１％（１／１４），总突变率为５７．１％（８／１４）。结果表明，ＳＣＬＣ组织中存在较高的ｐ５３基因突变率，故ｐ５３基因可能是人类肺癌发生的关键基因。

The Study of p53 Gene Mutation in Small Cell Lung Cancer

To explore the molecular mechanism of small cell lung cancer(SCLC),p53 gene mutation in 14 paraffin embedded SCLC tissue section was observed by immunohistochemical staining(LSAB method) and polymerase chain reaction and single strand conformation polymorphism(PCR SSCP) analysis.The results showed that the positive rate of immunohistochemical reaction was 64.3%(9/14).p53 gene point mutation in exon 5,6,7 and 8 was 21.4%(3/14);14.3%(2/14);14.3%(2/14);7.1%(1/14) respectively.The total mutation rate was 57.1%(8/14).It was suggested that comparatively high mutation rate of p53 gene exists in SCLC,which might be the crucial gene of the human lung carcinoma carcinogenesis.