体外培养大鼠胚胎脊髓神经元损伤前后的凋亡变化

目的研究体外培养脊髓神经元损伤前后的凋亡变化，进一步探讨中枢神经损伤的分子机制。方法取孕14天的Wistar大鼠脊髓神经元进行培养，划痕损伤神经元突起，对神经元损伤前后的凋亡变化进行观察。结果损伤前后体外培养脊髓神经元凋亡数量有明显差异，损伤前几乎未见凋亡的神经元，损伤后1天凋亡神经元最多，损伤后3天凋亡神经元也明显增多，损伤后5天凋亡神经元呈明显下降的趋势，损伤后7天凋亡神经元又开始增多。结论了解体外培养脊髓神经元损伤前后细胞凋的规律，为脊髓神经元损伤后给予干预措施的实验研究奠定了基础。

Apoptosis of primary spinal cord neurons of rat embryo before and following injury in vitro

Objective To investigate the apoptosis rate of neurons in vitro among the primary culture cells isolated from rat spinal cords before and after injury. Methods The spinal cord neurons of Wistar rats at 14 day gestation were isolated and cultured. The neuronal processes were then injured by cutting. At different time points after injury, TUNEL method was employed to detect the apoptotic neurons. Results Before injury, there was almost no apoptotic neuron. However, a large amount of apoptotic neurons were observed after the injury. The highest incidence of apoptosis appeared on the first day, and then it gradually reduced in the following days, but increased in amount again on the seventh day. Conclusion The results of the present experiment reveal the regularity of apoptosis of neurons before and after injury, and it provides a platform for further research regarding therapeutic intervention in the treatment of spinal cord injury.