脑梗死急性期患者血小板膜糖蛋白Ia C807T基因多态性和血小板功能

目的:探讨血小板膜糖蛋白(GP)Ia C807T基因多态性及与脑梗死急性期患者血小板功能的关系。方法:应用聚合酶链反应限制性片段长度多态性(PCR-RELP)方法检测97例急性期脑梗死患者(脑梗死组)及99例正常对照者(对照组)的GPIa C807T基因型,采用全血流式细胞术检测血小板P选择素(CD62P)表达率,采用比浊法检测花生四烯酸、二磷酸腺苷诱导的血小板最大聚集率(MARAA、MARADP)。结果:脑梗死组GPIa T等位基因频率高于对照组,差异有统计学意义(χ2=5.369,P<0.05);脑梗死组CD62P表达率、血小板MARAA、MARADP高于对照组(P<0.05)。对照组TT+CT基因型CD62P表达率和MARADP高于CC基因型(P<0.05);脑梗死组TT+CT基因型CD62P表达率和MARAA、MARADP与CC基因型差异无统计学意义(P>0.05)。结论:GPIaC807T可能是脑梗死发病的遗传危险因素。血小板活化和聚集功能增强可能是T等位基因促进脑梗死发病的机制之一。

Research of Platelet Glycoprotein Ia C807T Gene Golymorphism and the Function of Platelet in Acute Cerebral Infarction

Objective: To investigate the platelet glycoprotein Ia(GPIa) C807T gene polymorphism and the association of function of platelet in acute cerebral infarction. Method: By PCR-restriction fragment length polymorphism technique, GPIa C807T genotypes were detected in 97 patients with cerebral infarction(cerebral infarction group) and 99 healthy controls(control group). P-selectin(CD62P) was measured by flow cytometry, arachidonic acid-induced platelet maximal aggregation rate (MARAA),adenosine diphosphate-induced platelet maximal aggregation rate(MARADP ) were performed by turbidimetry. Results: The frequencies of GPIa T allele were significantly higher in the infarction group than in the control (χ2=5.369,P<0.05). The expression of CD62P and platelet MAR were significantly higher in the infarction group than in the control (P<0.05). The expression of CD62P and platelet MARADP in the control group with T allele were significantly higher than in those with CC genotype (P<0.05). The expression of CD62P and platelet MAR in the cerebral infarction group with T allele were not significantly higher than in those with CC genotype (P>0.05). Conclusion: GP Ia C807T allele might be an independent risk factor in the development of cerebral infarction. Enhanced platelet function might be one of the mechanisms of T allele in the pathogenesis of cerebral infarction.