| D-101大孔树脂分离纯化两面针总生物碱 |
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| 引用本文： | 陆世惠,龙盛京. D-101大孔树脂分离纯化两面针总生物碱[J]. 西北药学杂志, 2013, 28(1): 7-9 |
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| 作者姓名： | 陆世惠 龙盛京 |
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| 作者单位： | 广西医科大学药学院;右江民族医学院药学系 |
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| 基金项目： | 广西自然科学基金项目(编号0832158) |
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| 摘 要： | 目的研究大孔树脂分离纯化两面针总生物碱的工艺。方法用UV测定总生物碱含量来优化工艺参数,用TLC分析分离效果。结果以体积分数60%乙醇-5g·L-1盐酸提取液上D-101大孔树脂柱,流出液回收乙醇至45%,再上D-101大孔树脂柱,其流出液再回收乙醇至无醇味,再上D-101大孔树脂柱,分别用体积分数95%,80%和50%乙醇洗脱。产品总生物碱质量分数为18.07%～27.67%。结论该法既富集了总生物碱,又初步分离了不同极性成分。
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| 关 键 词： | 两面针 总生物碱 分离纯化 梯度吸附 大孔树脂 |
Separation and purification of total alkaloids from Zanthoxylum nitidum(Roxb.)DC.with D-101 macroporous resin |
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| LU Shihui,LONG Shengjing. Separation and purification of total alkaloids from Zanthoxylum nitidum(Roxb.)DC.with D-101 macroporous resin[J]. Northwest Pharmaceutical Journal, 2013, 28(1): 7-9 |
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| Authors: | LU Shihui LONG Shengjing |
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| Affiliation: | 1 (1.Pharmaceutical School,Guangxi Medical University,Nanning 530021,China;2.Pharmaceutical School,Youjiang Medical University for Nationalities,Baise 533000,China) |
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| Abstract: | Objective To explore a process to separate and purify total alkaloids from Zanthoxylum nitidum(Roxb.)DC.with macroporous resin.Methods UV was used to determine the total alkaloids while TLC was used to analyze the separation efficacy.Results Extract of volume fraction 60% alcohol-5 g·L-1 hydrochloric acid was absorbed and separated with D-101 macroporous resin.Then the alcohol in the effluent was recycled and reduced to 45%,and the concentrated solution was absorbed and separated with the resin.Finally,the alcohol in the effluent was recycled and reduced to no alcohol flavor,and the concentrated solution was absorbed and separated with the resin.Total alkaloids were eluted with 95%,80% and 50% alcohol,respectively.Mass fraction of total alkaloids in products was 18.07%-27.67%.Conclusion The process not only enriches total alkaloids,but also separates simply ingredients with different polarity. |
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| Keywords: | Zanthoxylum nitidum(Roxb.)DC. total alkaloids separation and purification gradient absorption macroporous resin |
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