薄层扫描法测定小儿健胃消食颗粒中熊果酸的含量

目的:建立小儿健胃消食颗粒中熊果酸含量的测定方法。方法:采用薄层扫描法,展开剂为氯仿-丙酮-冰醋酸(18∶2∶0.2),显色方法为10%硫酸乙醇溶液,于110℃下烘至斑点显清晰的紫红色,扫描方法为双波长反射式锯齿扫描,测定波长为520nm,参比波长为700nm,线性参数Sx=3,狭缝大小为0.4mm×0.4mm。结果:熊果酸进样量在2μg～10μg范围内与峰面积积分值线性关系良好(r=0.9996),平均回收率为97.8%(RSD=1.4%)。结论:本方法可用于小儿健胃消食颗粒的质量控制。

Content Determination of Ursolic Acid in Pedo-stomach-invigorating & Digestion-promoting Granule by TLC-scanning Method

OBJECTIVE:To establish a method for the determination of ursolic acid in pedo-stomach-invigorating&digestion-promoting granule.METHODS:The determination was performed by TLC-scanning method in which chloro-form-acetone-glacial acetic acid(18∶2∶0.2)was used as developing solvent,and10%sulphuric acid ethanol solution was taken as developer,which were baked under110℃until limpid prunosus spots were occurred,the scanning method was dual wavelength reflection zigzag scanning with detection wavelength at520nm,reference wavelength at700nm,linearity parameter Sx=3and slit size at0.4mm×0.4mm.RESULTS:Ursolic acid has a good linear relation with peak area score when its sample size was within the range of2μg～10μg(r=0.9996),the average recovery is97.8%(RSD=1.4%).CONCLUSION:This me_ thod can be served as a quality control for pedo-stomach-invigorating&digestion-promoting granule.