一种检测腹泻相关病毒的方法

目的 建立检测腹泻粪便标本中病毒病原的方法.方法 选取本实验室检测的杯状病毒、肠道腺病毒、轮状病毒、博卡病毒、星状病毒以及肠道病毒阳性标本各一份,通过非序列依赖性单-PCR扩增(Sequence-independent single primer amplification,SISPA)构建基因文库,从中筛查病毒基因片段.结果 六份标本中都可以得到相对应的病毒核酸序列.结论 本研究采用的方法可以检测到粪便标本中引起腹泻的相关病毒,为进一步研究检测目前尚未发现的病毒病原奠定基础.

A method of detect the virus which caused diarrhea

Objective Building a method which can examines virus pathogenic in gnstroenteritis excrement specimen.Methods Choosing six positive specimens which tested in our laboratory,include adenovirus,calicivirus,rotavirus,bocavirus,astrovirus and enterovirus.Through sequence-independent single primer amplification(SISPA) constructs a gene bank.Looks up the viral gene fragment in gene bank.Results Obtaining corresponding viral acid sequence in six specimens.Conclusion This research can examine enterovirns and the virus which cause diarrhea,It make a foundation for further studies the viral cause of disease which the examination not yet discovered at present.