重组人酸性成纤维细胞生长因子促进创伤愈合的研究

目的：研究重组人酸性成纤维细胞生长因子（rh-aFGF)对兔创伤的促愈合作用。方法：采用兔背部刀割伤模型，将rh-aFGF溶液隔日一次滴注于创面，用创面照像、透明膜描记称量法记录伤后第4、8、12、16天创面面积，用注水法测量伤腔容积，伤后第8、16天取创面组织，观察创面的病理学变化，包括肉芽组织生长与再上皮化情况。结果：rh-aFGF可明显加速兔皮肤创伤的愈合，使创面面积明显缩小（P＜0．05），使伤腔容积明显减少（P＜0．05）。组织学检查：rh-aFGF组创面伤后8天成纤维细胞生长活跃、数量多，其毛细血管胚芽与成纤维细胞数量显著多于对照组；伤后16天，创面收缩与再上皮化明显，新生上皮向创面中心爬行较快。结论：rh-aFGF对兔背部刀伤创面有明显的促修复作用。

Study of Recombinant Human Acidic Fibroblast Growth Factor on Improving Wound Healing in Rabbits

AIM To study the effects of recombinant human acidic fibroblast growth factor (rh aFGF) on improving wound healing of rabbit skin. METHODS Taking knife wound as model, each rabbit had 6 round shaped wound (18 mm in diameter), rh aFGF (180 U/cm 2, 60 U/cm 2, 20 U/cm 2) was dressed on the surface of wound at intervals of two days. The area and depth of cut were measured at different time, and the histology was observed on the 8th and 16th day. RESULTS The wound area decreased obviously in treatment groups (60 U/cm 2, 20 U/cm 2) on the 4th, 8th and 12th day. The wound depth was shallower in treatment group (60 U/cm 2) on the 4th, 8th and 12th day than that in control group. The histomorphometric results indicated that fibroblastic cells had flourish growth, the quantity of capillary vessel embryo and fibroblastic cells in treatment group were manifold than that in control group on the 8th day after injury. CONCLUSION The knife wound healing on rabbits back was improved significantly by daubing rh aFGF.