吡格列酮对db/db小鼠骨骼肌蛋白酪氨酸磷酸酶1B表达的影响

目的 探讨吡格列酮对db/db小鼠骨骼肌蛋白酪氨酸磷酸酶1B( protein tyrosine phosphatase 1B,PTP1B)表达水平的影响.方法 将20只4周龄db/db小鼠随机分为两组(吡格列酮组和db/db对照组),每组10只,分别给予吡格列酮10mg/kg.d和安慰剂灌胃.另设10只同周龄db/m小鼠,给予安慰剂灌胃作为非糖尿病对照(db/m对照组).每周监测体重、血糖,4周后用蛋白印迹法检测各组小鼠骨骼肌组织中PTP1 B蛋白含量.结果 db/db组小鼠骨骼肌PTP1B表达显著高于db/m组,给予吡格列酮干预,血糖、胰岛素抵抗指数显著低于db/db组(P＜0.05),骨骼肌PTP1B表达水平亦显著降低(P＜0.05).结论吡格列酮改善胰岛素抵抗,可能与降低骨骼肌PTP1B蛋白表达有关.

The Effects of Pioglitazone on Expression of Protein Tyrosine Phosphatase 1B in Muscle of db/db Mice

Objective To explore the effects of pioglitazone on expression of protein tyrosine phosphatase 1B in muscle of db/db mice.Methods Twenty db/db mice aged 4 weeks were randomized to pioglitazone group(PIO group) and db/db group,fed with 10mg/kg·d pioglitazone and placebo respectively.Ten age-matched db/misty mice were concurrently treated with placebo as non-diabetic control group(db/m group).Body weight and blood glucose were measured every week.After 4 weeks′treatment,the expression of PTP1B in muscle of mice were determined with Western blotting.Results The expression of PTP1B in muscle of db/db mice significantly increased compared with db/m(P<0.05).Pioglitazone treatment significantly decreased fasting blood glucose and HOMA-IR.The expression of PTP1B in muscle of pioglitazone-treated mice obviously decreased compared with db/db group(P<0.05).Conclusion Pioglitazone treatment could improve insulin resistance possibly by reducing the expression level of PTP1B in the muscle.