重症急性胰腺炎大鼠Treg及内毒素的变化

目的探讨CD4+CD25+调节性T细胞(Treg)及肠源性内毒素(ET)在重症急性胰腺炎中的作用。方法 60只SD大鼠随机分成假手术组(SO)和SAP组,成功造模后3、6、12h取大鼠胰腺行病理学检查;流式细胞术检测外周血Treg水平;鲎试剂法测血浆内毒素水平。结果 SAP组胰腺病理学评分随病程进展升高,血浆ET,外周血Treg水平逐渐明显升高(P<0.05)。结论 Treg和ET在重症急性胰腺炎发生发展中可能发挥重要作用。

The changes of CD4~+CD25~+ regulatory T cells in peripheral blood and intestinal endotoxin in severe acute pancreatitis rats

Objective To investigate the role of CD4+CD25+ regulatory T cells(Treg) in peripheral blood and intestinal endotoxin(ET) in severe acute pancreatitis(SAP) rat.Methods Sixty male SD rats were randomly allocated into sham operation group(SO group) and severe acute pancreatitis group(SAP group).The histopathological changes of the pancreas were observed by lightmicroscopy at 3-,6-,12-hour after operation for both SO group an SAP group,the proportion of Treg among peripheral lymphocytes was determined by Flow Cytometry(FCM),limulus reagent method is used to determine ET content in plasma.Results Histopathologic score revealed the occurrence of pancreatic inflammation and pathological changes in SAP groups.The percentage of Treg and plasma ET levels in SAP groups significantly increased comparing to the SO groups(P <0.05).Conclusion The high plasma levels of ET and Treg in peripheral blood may play an important role in SAP.