丙酮酸乙酯对脓毒症大鼠肝细胞线粒体的保护作用

目的研究丙酮酸乙酯（ethyl pyruvate，EP）对脓毒症大鼠肝细胞线粒体的保护作用。方法采用盲肠结扎穿孔（cecal ligation-perforation，CLP）法制作大鼠脓毒症模型，60只大鼠分3组：假手术组（n=20）、CLP模型组（n=20）、CLP＋EP治疗组（n=20），检测肝细胞线粒体超微结构及功能变化。结果电镜扫描结果显示CLP模型组肝细胞线粒体发生明显结构损伤改变，CLP＋EP治疗组肝细胞线粒体病变较轻。比较分析CLP模型组与CLP＋EP治疗组的超微结构参数表明：两组的比表面积及形态因子数值间差异无统计学意义（0．027vs.0．029，1．162vs．1．300），但CLP＋EP治疗组的中位数较CLP模型组更接近正常；与CLP模型组比较，CLP＋EP治疗组线粒体横截表面积减小，差异有统计学意义[（0．641±0．460）vs.（0．231±0．110）μm^2，P〈0．01]。与假手术组比较。CLP模型组肝细胞线粒体呼吸链复合体Ⅰ活性下降42％；CLP＋EP治疗组的线粒体呼吸链复合体Ⅰ活性仅下降12％（P〈0．05）。结论EP对脓毒症大鼠肝细胞线粒体结构及功能有一定保护作用。

Protection of hepatocyte mitochondria by ethyl pyruvate in septic rat

Objective To investigate the protection of hepatocyte mitochondria by ethyl pyruvate(EP) in septic rats.Methods Cecal ligation-perforation(CLP) models were made in rat as septic model.Total of 60 rats were divided medially into three groups:sham group,CLP group and CLP + EP treatment group.Hepatocyte mitochondria ultrastructure and function were detected in different groups.Results Mitochondria ultrastructure in CLP group showed significant damage,whereas mitochondria less in change CLP + EP treatment group.Compared the ultrastructural data between CLP group and CLP + EP treatment group,it didn’t show significant difference in specific surface area and modality factor between the two groups(0.027 vs.0.029,1.162 vs.1.300).But median in CLP + EP treatment group still approach to normal than CLP group.Surface area of mitochondria significant decreased in CLP + EP treatment group than CLP group(0.231 ± 0.110 vs.0.641 ± 0.460 μm2),P < 0.001.Compared with the sham group,the activity of mitochondrial respiratory chain I were significant decreased by 42% in CLP group,while the activity in CLP + EP treatment group decreased by 12%(P < 0.05).Conclusion Ethyl pyruvate shows protective effect of hepatocyte mitochondria in septic rat.