| PARP-1抑制剂AG014699对人卵巢癌细胞株SKOV3增殖及凋亡的影响 |
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| 引用本文： | 徐晶晶,摆扬,王海琳. PARP-1抑制剂AG014699对人卵巢癌细胞株SKOV3增殖及凋亡的影响[J]. 中国现代医药杂志, 2012, 14(3): 18-20 |
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| 作者姓名： | 徐晶晶 摆扬 王海琳 |
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| 作者单位： | 1. 730000,兰州大学第一临床医学院妇产科
2. 甘肃省人民医院妇产科 |
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| 摘 要： | 目的探讨PARP-1抑制剂AG014699对人卵巢癌细胞株SKOV3`增殖及凋亡的影响。方法①采用MTT法测定AG014699在不同浓度、不同时间对人卵巢癌细胞株SKOV3增殖作用的影响;②用流式细胞仪测定AG014699在50μmol/L浓度下对人卵巢癌细胞株SKOV3作用24h后对细胞周期及凋亡的影响。结果①MTT测定:在24、48、72h时AG014699在10μmol/L分别测得的OD值与对照组相比,P<0.05,有统计学差异;②流式细胞仪测定:AG014699在50μmol/L浓度下对SKOV3作用24h能明显改变细胞的周期分布,诱导细胞凋亡。结论 AG014699能明显抑制人卵巢癌细胞株SKOV3的增殖并诱导其凋亡。
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| 关 键 词： | PARP-1 AG014699 人卵巢癌细胞株SKOV3 细胞周期 凋亡 |
Effects of Poly(ADP-ribose)Polymerase-1 inhibitor AG014699 on growth and apoptosis of human ovary cancer cell line SKOV3 |
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| Xu Jingjing , Bai Yang , Wang Hailin. Effects of Poly(ADP-ribose)Polymerase-1 inhibitor AG014699 on growth and apoptosis of human ovary cancer cell line SKOV3[J]. Modern Medicine Journal of China, 2012, 14(3): 18-20 |
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| Authors: | Xu Jingjing Bai Yang Wang Hailin |
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| Affiliation: | .The 1st Clinical Medical Science University of Lanzhou University,Lanzhou 730000 |
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| Abstract: | Objective To explore the effects of Poly(ADP-ribose)Polymerase-1 inhibitor AG014699 on the cell proliferation and apoptosis of human ovary cancer cell line SKOV3.Methods ①Growth inhibition rate of SKOV3 cells was measured with MTT method;②Apoptotic rate and cell cycle of the cells at 50μmol/L were inspected by flow cytometery(FCM)after 24h.Results ①At 10μmol/L,AG014699 inhibited the growth of ovary cancer cells SKOV3 at 24,48 and 72h,P<0.05 compared control group.②At 50μmol/L,AG014699 induced the apoptosis of the human ovary cancer cellline SKOV3 at 24h,and changed the cell cycle.Conclusion AG014699 significantly inhibits the growth of ovary cancer cells SKOV3 and induces apoptosis. |
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| Keywords: | Poly(ADP-ribose)Polymerase-1 AG014699 Human ovary cancer cell line SKOV3 Cell cycle Apoptosis |
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