纳米雄黄对MCF-7乳腺癌干细胞的体外抑制作用

目的 研究纳米雄黄对乳腺癌干细胞的体外抑制作用.方法 以人乳腺癌MCF-7亲本细胞为对象,采用无血清培养法培养获得乳腺癌干细胞.以阿霉素(1 mg/L)为阳性对照,以相同质量浓度的水飞雄黄为参照,采用CCK-8法检测纳米雄黄对MCF-7亲本细胞及干细胞增殖的影响;借助悬浮球形成及分化实验、划痕实验、Transwell侵...

In vitro inhibitory effects of realgar nanoparticles on MCF-7 breast cancer stem cells

OBJECTIVE To study in vitro inhibitory effects of realgar nanoparticles on breast cancer stem cells.METHODS Human breast cancer MCF-7 parent cells were selected as subjects and cultured by serum-free culture to obtain breast cancer stem cells.Using adriamycin(1 mg/L)as positive control,same concentration of water-processed realgar as reference,the effects of realgar nanoparticles on the proliferation of MCF-7 parent cells and stem cells were detected by CCK-8 method.The effects of realgar nanoparticles on the formation of mammosphere,the ability of differentiation,migration and invasion,the proportion of CD44⁺/CD24^-subgroup in breast cancer stem cells were detected by mammosphere formation and differentiation experiment,scratch experiment,Transwell invasion experiment and flow cytometry.Western blot assay was used to detect the expression of proteins related to epithelial mesenchymal transformation pathway(E-cadherin and vimentin)in breast cancer stem cells.RESULTS The survival rates of MCF-7 parent cells and stem cells(except for breast cancer stem cells in both 1 mg/mL groups)in 1,5,10,40,60,80 mg/L groups of water-processed realgar and realgar nanoparticles were significantly lower than blank control group(P<0.01).The number of mammosphere(>20 stem cells)in 1,2.5,5,10 mg/L groups of water-processed realgar and realgar nanoparticles was significantly lower than blank control group(P<0.01);the volume of mammosphere decreased and the differentiated adherent cells decreased;the healing rate of wound,relative invasion rate(except for water-processed realgar 1 mg/L group)and the proportion of CD44⁺/CD24^-subgroup were significantly lower than blank control group(P<0.01).The expressions of E-cadherin in 2.5,10 mg/L groups of water-processed realgar and realgar nanoparticles was significantly higher than blank control group,and the expressions of vimentin was significantly lower than those in blank control group(P<0.01).The above effects of realgar nanoparticles were generally better than those of water-processed realgar with the same mass concentration(P<0.01).CONCLUSIONS Compared with water-processed realgar with the same mass concentration,realgar nanoparticles can significantly inhibit the proliferation of breast cancer stem cells,the formulation and differential ability of mammosphere,and reduce the proportion of CD44⁺/CD24^-subgroup.The effect may be associated with the inhibition of migration and invasion of breast cancer stem cells by inhibiting the expression of proteins related to epithelial mesenchymal transformation pathway.