乳腺癌中Nupr1蛋白及mRNA表达及临床意义

目的 检测Nupr1蛋白及mRNA在乳腺癌中的表达,探讨其与乳腺癌的关系。方法 选取手术切除的228例乳腺癌组织,以60例乳腺良性病变为对照组,用免疫组化法检测癌组织Nupr1蛋白的表达情况,用实时荧光定量PCR法检测组织中Nupr1 mRNA 的表达,分析Nupr1的表达与乳腺癌临床病理特征的关系。结果 乳腺癌组织Nupr1蛋白及mRNA水平均显示高表达,二者增高趋势趋于一致,乳腺癌组织Nupr1高表达率均高于对照组;Nupr1高表达率与乳腺癌分期、分子分型相关(P<0.05),与月经状态、组织学类型、组织学分级及肿瘤大小无关(P>0.05)。结论 Nupr1 mRNA在乳腺良性病变-乳腺癌前病变-乳腺癌中表达逐渐增高,推测此基因可能在乳腺癌发生及进展过程中起了重要作用;在mRNA水平及蛋白水平表达的一致性进一步说明了此基因在乳腺癌发生发展中起了重要作用;Nupr1高表达与预后不良有关,检测Nupr1的表达情况可作为乳腺癌预后判断的有用指标,并可为肿瘤靶向治疗提供新思路。

The expression of Nupr1 in breast cancers and it's clinical significance

Objective To study the expression of Nupr1(including mRNA and protein)in breast cancers and explore the relationship between Nupr1 expression and the clinical/pathological characteristics in breast cancer. Methods The expression of Nupr1 protein was detected with immunohistochemistry in 228 cases of breast cancer and 60 cases of benign breast lesions as control group. The expression of Nupr1 mRNA was detected with real-time fluorescence quantitative PCR. The relationships between Nupr1 expression and clinical pathological characteristics of breast cancer were analyzed. Results The level of Nupr1 protein and mRNA in breast cancer showed high expression, which tended to be consistent. The high expression rate of Nupr1 in breast cancer was significantly higher than that in the control group(P<0.05). The high expression rate of Nupr1 was significantly correlated with breast cancer stage and molecular type(P<0.05), but not with menstrual state, histological type, histological grade and tumor size(P>0.05). Conclusion The expressionof Nupr1 mRNA in benign breast lesions-precancerous breast lesions-breast cancer is increasing gradually. It is speculated that Nupr1 may play an important role in the occurrence and progression of breast cancer. The consistent expression of Nupr1 at mRNA level and protein level suggested that Nupr1 plays an important role in the occurrence and development of breast cancer. The high expression of Nupr1 is related to poor prognosis. The detection of Nupr1 expression can be used as breast cancer detection The useful indexes of cancer prognosis can provide new ideas for tumor targeted therapy.