实时荧光核酸恒温扩增检测技术在泌尿生殖道淋病中的应用

目的探讨实时荧光核酸恒温扩增检测技术（SAT）检测泌尿生殖道淋病的灵敏度、特异度以及监测疗效的实用性。方法回顾性分析12816例因怀疑泌尿道淋球菌感染需行淋球菌检测受试者临床资料，其中淋球菌培养检测6323例，基于SAT开发的淋球菌核酸检测试剂盒（NG-SAT）检测7107例，比较2种检测方法的阳性率；再以淋球菌培养法为金标准，计算同时行以上2种检测的614例患者NG-SAT检测尿液样本的灵敏度和特异度；对2种检测结果有差别的标本，进行荧光定量DNA杂交及聚合酶链反应法（FQ-PCR）复测与分析；对检测结果阳性的患者进行头孢曲松治疗，待症状消失后3d再次行以上2种检测。结果NG-SAT检测尿液标本的灵敏度为100.0%，特异度为98.2%。2种检测结果有差别的标本7份，均为SAT检测阳性、淋球菌培养阴性；FQ-PCR复测结果显示6份阳性、1份阴性。179例患者经头孢曲松治疗后，NG-SAT检测阳性15例，淋球菌培养阳性7例。结论NG-SAT检测尿液中淋球菌简便、准确，且能准确判断预后，值得临床推广应用。

Detection of urinary Neisseria Gonorrhoea infection with simultaneous amplification and testing technique

Objective To evaluate the application of real-time simultaneous amplification and testing (SAT) technique in detection of urinary Neisseria Gonorrhoea (NG) infection. Methods Total 12 816 patients with suspected urogenital tract infection were tested in our hospital between February 2012 and November 2015, of whom 6 323 were tested by bacterial culture and 7 107 were tested by NG-SAT, and 614 were test by both methods. Swab and urine samples were collected for tests, the swab was tested by liquid culture, while urine samples by SAT. Samples with inconsistent results of two tests were retested by PCR. Results The positive rates of liquid culture and SAT were 35.9% and 37.7%, respectively. The specificity and sensitivity of SAT were 100.0% and 98.2% . In 7 patients with positive SAT and negative culture results, PCR showed 6 of them were positive. Conclusion Detection of NG with SAT technique has high sensitivity and specificity, which provides a new diagnostic method for urinary NG infection.