有氧运动联合褪黑素对二型糖尿病大鼠骨质疏松影响的实验研究

目的观察有氧运动和褪黑素对二型糖尿病大鼠骨质疏松的影响。方法成年雌性SD大鼠80只,6周龄,随机分为安静对照组(N)、糖尿病对照组(D)、正常有氧运动组(E)、糖尿病有氧运动组(DE)、正常褪黑素组(M)、糖尿病褪黑素组(DM)、正常有氧运动褪黑素组(EM)、糖尿病有氧运动褪黑素组(DEM),观察各组大鼠体重、脊椎骨以及左右股骨骨密度(BMD)、观察大鼠血糖、血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、血清总钙(Ca)、无机磷(P)和甲状旁腺素(PTH)的变化。结果与N组相比,E、M和EM组大鼠体重、血糖、血Ca、血P和血PTH无明显变化(P0.05),血清SOD、GSH-Px水平、腰椎和左右股骨BMD显著升高(P0.05),血清MDA水平显著降低(P0.05),D组大鼠体重、血清SOD、GSH-Px水平、血Ca、腰椎和左右股骨BMD显著降低(P0.05),血糖、血清MDA和血PTH水平显著升高(P0.05),血P无明显变化(P0.05);与D组比较,DE、DM组大鼠体重、血清SOD、GSH-Px水平、血Ca、腰椎和左右股骨BMD显著升高(P0.05),血糖、血清MDA和血PTH水平显著降低(P0.05),血P无明显变化(P0.05),有氧运动和褪黑素同时干预效果更好。结论有氧运动和褪黑素均能改善糖尿病骨质疏松,且两者联合干预的效果更加显著,其可能与通过提高糖尿病大鼠的抗氧化应激能力,调节糖的代谢从而有效地的降低血钙和PTH,改善BMD来缓解骨质疏松有关。

Experimental study of the effect of aerobic exercise combined with melatonin on osteoporosis in diabetic rats

Objective To investigate the effect aerobic exercise combined with melatonin on osteoporosis in rats with type II diabetes. Methods Eighty 6-week-old adult female SD rats were randomly divided into the control group (N), diabetic control group (D), normal aerobic exercise group (E), diabetes aerobic exercise group (DE), normal faded melanin group (M), diabetes melatonin group (DM), normal aerobics melatonin group (EM), and diabetes aerobic exercise melatonin group (DEM). Body weight, femur bone mineral density (BMD) of the spine and the hips, blood glucose, serum malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), serum total calcium (Ca), inorganic phosphorus (P), and parathyroid hormone (PTH) were observed in rats of each group. Results Compared with those in group N, body weight, blood glucose, blood Ca, P, and serum PTH blood in group E, M and EM rats were not significantly changed (P>0.05), but serum SOD, GSH-Px, lumbar and femoral BMD increased significantly (P<0.05), serum MDA level decreased significantly (P<0.05). Body weight, serum SOD, GSH-Px, blood Ca, lumbar spine and femoral BMD decreased significantly (P<0.05), but blood glucose, MDA, and serum PTH increased significantly in group D (P<0.05), and blood P was not significant changed (P>0.05). Compared with those in group D, body weight, serum SOD, GSH-Px, blood Ca, BMD of the left and right femur and lumbar spine increased significantly (P<0.05), but blood glucose, serum MDA and serum PTH decreased significantly (P<0.05), and no significant change in blood P (P>0.05) in group DE and DM. The simultaneous intervention with both aerobic exercise and melatonin was better. Conclusion Aerobic exercise and melatonin relieve diabetic osteoporosis, and the effect is more significant with the joint intervention. The effect may be associated with the increase of anti-oxidative stress ability, regulation of glucose metabolism in order to effectively reduce serum calcium and PTH, resulting in improving BMD to alleviate osteoporosis.