人参皂甙Rg3对胰腺癌SW-1990细胞皮下移植瘤血管生成拟态影响的研究

目的研究人参皂甙Rg3对胰腺癌SW-1990细胞皮下移植瘤生长及血管生成拟态的影响。方法用胰腺癌SW-1900细胞株建立裸鼠胰腺癌皮下移植瘤模型，分成4组：0.9%氯化钠溶液对照组、5mg/kg人参皂甙Rg3组、10mg/kg人参皂甙Rg3组、20mg/kg人参皂甙Rg3组，每组7只。各组裸鼠予腹腔注射给药，隔天1次，给药4周。给药结束后3d处死裸鼠，检测各组移植瘤的体积和重量；CD31/PAS染色观察移植瘤血管生成拟态的变化；荧光定量PCR和Westernblot检测各组移植瘤钙粘蛋白（VE-Cadherin）、络氨酸激酶受体2（EphA2）、基质金属蛋白2（MMP-2）、基质金属蛋白9（MMP-9）mRNA和蛋白表达水平。结果与对照组比较，20mg/kg人参皂甙Rg3组裸鼠胰腺癌皮下移植瘤体积减小、重量减轻（均P＜0.05）。与对照组比较，5mg/kg人参皂甙Rg3组、10mg/kg人参皂甙Rg3组、20mg/kg人参皂甙Rg3组裸鼠胰腺癌皮下移植瘤拟态、正常血管生成均减少（均P＜0.05），VE-Cadherin、EphA2、MMP-2、MMP-9mRNA和蛋白表达水平均降低（均P＜0.05）。结论人参皂甙Rg3可抑制胰腺癌SW-1900细胞皮下移植瘤生长和血管生成拟态形成，可能是通过调控VE-Cadherin、EphA2、MMP-2、MMP-9的表达发挥抗肿瘤作用。

Effect of ginsenoside Rg3 on growth and vasculogenic mimicry of implanted human pancreatic cancer in nude mice

Objective To investigate the effects of ginsenoside Rg3 on growth and vasculogenic mimicry of implanted human pancreatic cancer in nude mice. Methods Human pancreatic cancer SW-1990 cells were inoculated subcutaneously in nude mice. The tumor bearing nude mice were divided into four groups with 7 mice in each group. The animals were injected intraperitoneally with saline control, 5 mg/kg, 10mg/kg and 20mg/kg ginsenosides Rg3 for 4 wk, respectively. The animals were sacrificed 3d after completion of the treatment. The weight and size of xenografts were measured, the vasculogenic mimicry was observed by immunohistochemistry with CD31/PAS staining, and the mRNA/protein expressions of VE-Cadherin, EphA2, MMP-2, MMP-9 were detected by fluorescent RT-PCR and Western blot, respectively. Results Ginsenosides Rg3 significantly inhibited weight and size of transplanted tumors; and inhibited vasculogeic mimicry. The expression of VE-Cadherin, EphA2, MMP-2 and MMP-9 mRNA and protein were down-regulated. Conclusion Rg3 can inhibit vasculogenic mimicry of transplanted human pancreatic cancer in nude mice, which may be associated with the down-regulation of VE-Cadherin, EphA2, MMP9 and MMP2 expression.