| 内毒素对牙龈成纤维细胞增殖活性及分泌基质金属蛋白酶的影响 |
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| 引用本文： | 于艳玲,许丽华,王俊霞,尤红煜,杨冬茹.内毒素对牙龈成纤维细胞增殖活性及分泌基质金属蛋白酶的影响[J].现代口腔医学杂志,2010,24(1):60-63. |
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| 作者姓名： | 于艳玲 许丽华 王俊霞 尤红煜 杨冬茹 |
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| 作者单位： | 1. 秦皇岛海港医院口腔内科
2. 河北医科大学口腔医院,石家庄,050017 |
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| 基金项目： | 河北医科大学青年基金 |
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| 摘 要： | 目的观察内毒素对牙龈成纤维细胞增殖活性及分泌基质金属蛋白酶-1,3量的影响,探讨基质金属蛋白酶在牙周炎致病机制中的可能作用。方法通过改良酶消化组织块法进行牙龈成纤维细胞的原代培养,用内毒素对其进行刺激,运用MTT法观察内毒素对牙龈成纤维细胞增殖活性的影响,通过免疫组化法检测内毒素对牙龈成纤维细胞分泌基质金属蛋白酶-1,3的影响。结果MTT结果显示,低浓度内毒素在短期内可以促进牙龈成纤维细胞的增殖,但随着时间的延长,也表现为对细胞的毒性作用,抑制其增殖;高浓度内毒素抑制牙龈成纤维细胞的增殖;免疫组化研究结果表明,未受LPS刺激的牙龈成纤维细胞MMP-1,3表达弱阳性,受刺激后阳性表达增强,在12h时达到顶峰,且MMP-3的整体表达较MMP-1弱。结论LPS可以影响牙龈成纤维细胞的增殖活性;LPS可以促进牙龈成纤维细胞对基质金属蛋白酶的分泌,加快牙周细胞外基质的降解,加速牙周炎的进程。
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| 关 键 词： | 基质金属蛋白酶 人牙龈成纤维细胞 内毒素 细胞原代培养 牙周炎 |
Influence of Lipopolysaccharide on the proliferation rate and the expression of MMPs by the human gingival fibroblasts |
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| YU Yanling,XU Lihua,WANG Junxia,et al..Influence of Lipopolysaccharide on the proliferation rate and the expression of MMPs by the human gingival fibroblasts[J].Journal of Modern Stomatology,2010,24(1):60-63. |
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| Authors: | YU Yanling XU Lihua WANG Junxia |
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| Institution: | YU Yanling,XU Lihua,WANG Junxia,et al.Hebei Medical University,Shijiazhuang 050017 |
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| Abstract: | Objective To investigate the influence of lipopolysaccharide(LPS) stimulation on the proliferation rate of human gingival fibroblasts(HGFs)and the expression of MMP-1 and MMP-3 by HGFs,so to further understand the role of metalloproteinases(MMPs) in the process of peridontitis.Methods HGFs were cultured by using improved enzyme digestion tissue culture method,and were then challenged by LPS,the proliferation activity of HGFs was examed by MTT assay;the expression of MMP-1 and MMP-3 was examed by immunocytoc... |
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| Keywords: | Metalloproteinases (MMPs) Human gingival fibroblasts (HGFs) Lipopolysaccharide (LPS) Primary cell culture Periodontitis |
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