| Cloning and identification of NS5A TP2gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A |
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| 基金项目： | Supported by the National Natural Science Foundation of China,No.C03011402,No.C30070690 and the 9th Five-year Plan Period Research and Development Foundation of PLA,No.98D063 and the Start-up for Students Studying Overseas of PLA,No.98H038 and the 10th F |
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| 摘 要： | AIM:To clone,identify and study new NS5ATP2 gene andits spliced variant transactivated by hepatitis C virus non-structural protein 5A.METHODS:On the basis of subtractive cDNA library of genestransactivated by NS5A protein of hepatitis C virus,the codingsequence of new gene and its spliced variant were obtainedby bioinformatics method.Polymerase chain reaction(PCR)was conducted to amplify NS5ATP2 gene.RESULTS:The coding sequence of a new gene and itsspliced variant were cloned and identified successfully.CONCLUSION:A new gene has been recognized as thenew target transactivated by HCV NS5A protein.These resultsbrought some new clues for studying the biological functionsof new genes and pathogenesis of the viral proteins.
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