效应蛋白酶受体-1在鼻咽癌组织中的表达及其与细胞增殖和凋亡的关系

目的：探讨凝血蛋白酶因子Xa（FXa）的效应蛋白酶受体-1（EPR-1）在鼻咽癌（NPC）组织中的表达及其与细胞增殖、凋亡之间的关系。方法：用免疫组织化学SP法检测NPC组织、癌旁交界组织各42例及鼻咽部黏膜慢性炎症组织30例中EPR-1、增殖细胞核抗原Ki-67的表达，以Ki-67代表细胞增殖指数（PI）；用TdT酶介导的生物素化dUTP缺口末端标记技术（TUNEL法）检测凋亡指数（AI）并进行统计学分析。结果：EPR-1在NPC的组织、癌旁交界组织、鼻咽部黏膜慢性炎症组织中的阳性表达率分别为76．1％、90．5％、100．0％，3组间差异有统计学意义（均P〈0．01），与AI呈正相关（r=0．551，P〈0．01），与PI呈负相关（r=-0．338，P〈0．05）。结论：EPR-1促进细胞凋亡，抑制细胞增殖，为以Survivin为靶向的NPC治疗开创新的途径。

The role of EPR-1 in proliferation and apoptosis in nasopharyngeal carcinoma

Objective:To investigate the relationship between the expression of effector cell protease receptor-1(EPR-1) of coagulation serine protease factor Xa,proliferative index (PI) ki-67 and apoptotic index(AI) in nasopharyngeal carcinoma.Method:Using immunohistochemical S-P staining and terminal deoxynucleotidyl transferase mediate dUTP nick and labeling (TUNEL) technique, the expression of EPR-1, ki-67 and AI was detected in the paraffin-embedded specimens including 42 nasopharyngeal carcinoma tissues,42 normal nasopharyngeal tissues adjacent to the tumors tissues and 30 chronic nasopharyngitis tissues.Result:The positive rate of EPR-1 expression in the NPC tissues, normal nasopharyngeal tissues adjacent to the tumors and chronic nasopharyngeal tissues was 76.1%,90.5%,100.0%,respectively. EPR-1 was positively correlated with AI and negatively with ki-67.Conclusion:EPR-1 play a role in increase the apoptosis and decrease the proliferation of cell.These suggest that EPR-1 may be a potential therapy for NPC.