人舌鳞癌TCA8113细胞在不同温度下nm23-H1基因的表达及其意义

目的:通过测定肿瘤细胞内nm23-H,基因的表达情况,探讨高温治癌与转移抑制基因nm23-H_1之间的关系,以期阐明高温抑制肿瘤侵袭及转移的机理。方法:将人舌鳞癌细胞株Tca-8113细胞分为37℃对照组和43℃实验组,43℃组又分为10、20、30和40min共4个时间亚组。经相应的温度和时间处理后,通过MTT法、免疫组织化学技术及RT-PCR等方法对细胞进行观察、分析,以研究加热后舌癌细胞的生物学行为及nm23-H_1表达量的改变。结果:加温能抑制Tca-8113细胞的增殖活性,在43℃随着加热时间的延长,细胞中nm23-H_1表达量增加,并且在加热30min时达到最高.而在加热40min时有所下降.此变化不随加热后培养时间的延长而改变。结论:高温治疗可能是通过增加细胞中转移抑制基因nm23-H_1表达量来抑制肿瘤细胞的侵袭、转移能力;43℃加热30min可能是临床治疗的理想位点。

Expression of the Antimetastatic Gene m23-H1 in Human Tongue Cancer Cell in Relation with Hyperthermia

Objective:To investigate the relation ship between hyperthermia treatment of tongue cancer cells and the expression of gene nm23-H_1.Methods:Tongue squamous carcinoma cells(Tca-8113)was heated and the quantities of nm23-H_1 were detected and analyzed through MTT assay,immunohistochemistry and RT-PCR.Results:Hyperthermia suppressed cell proliferation.In 43℃,temperature induced a time-dependent gene expression that peaked at 30 min.Gene expression declined at 40℃and remained constant throughout the duration.Conclusion:Hyperthermia can suppress cancer cell invasion and metastasis through the synthesis of nm23-H_1 metastasis supressor gene.Cancer cells heated at 43℃for 30 min may be ideal.