| 人正常子宫内膜蛋白质表达图谱的初步建立 |
| |
| 引用本文： | 刘海元,郎景和,冷金花,孙大为,朱兰,刘珠凤. 人正常子宫内膜蛋白质表达图谱的初步建立[J]. 生殖医学杂志, 2008, 17(3): 192-195 |
| |
| 作者姓名： | 刘海元 郎景和 冷金花 孙大为 朱兰 刘珠凤 |
| |
| 作者单位： | 中国医学科学院,北京协和医学院,北京协和医院妇产科,北京,100730 |
| |
| 摘 要： | 目的通过双相电泳方法建立正常子宫内膜组织的蛋白质表达图谱。方法收集正常卵泡期子宫内膜组织5份,采用双相电泳建立子宫内膜组织的蛋白质表达谱;利用凝胶染色技术和图像分析软件进行蛋白质点的识别和检测;随机选择5个蛋白质点,应用基质辅助激光解析离子化飞行时间质谱(MALDI-TOF-MS)和生物信息学方法进行蛋白质的鉴定。结果以固相pH梯度(IPG)胶条pH=4~9进行第一相等电聚焦,以12.5%SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)为第二相,成功建立了正常卵泡期子宫内膜组织蛋白质双相电泳图谱,平均含(523±41)个蛋白点,匹配率81.89%。通过MALDI-TOF-MS对5个蛋白质点进行了鉴定。结论成功建立了人正常子宫内膜蛋白质双相电泳图谱,并应用质谱技术进行了部分蛋白质点的鉴定,为进一步构建人子宫内膜蛋白质表达数据库提供了基础,并为研究子宫内膜生理功能和相关疾病提供了一种新的研究方法。
|
| 关 键 词： | 子宫内膜 双相电泳 基质辅助激光解吸离子化飞行时间质谱 蛋白质组 |
| 文章编号： | 1004-3845(2008)03-0192-04 |
| 修稿时间： | 2008-03-26 |
A pilot study in the establishment of normal human endometrium proteomic profile |
| |
| LIU Hai-yuan,LANG Jing-he,LENG Jin-hua,SUN Da-wei,ZHU Lan,LIU Zhu-feng. A pilot study in the establishment of normal human endometrium proteomic profile[J]. Journal of Reproductive Medicine, 2008, 17(3): 192-195 |
| |
| Authors: | LIU Hai-yuan LANG Jing-he LENG Jin-hua SUN Da-wei ZHU Lan LIU Zhu-feng |
| |
| Affiliation: | LIU Hai-yuan,LANG Jing-he,LENG Jin-hua,SUN Da-wei,ZHU Lan,LIU Zhu-fengDepartment of Obstetrics & Gynecology,Peking Union Medical College Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100730 |
| |
| Abstract: | Objectives: To establish the protein profile of human endometrium by two-dimensional electrophoresis.Methods: Five normal endometria in proliferative phase were collected during trans-abdominal hysterectomy.The whole protein extracts from endometrium were separated using two-dimensional gel electrophoresis(2 DE).Protein spots were stained by Comassie blue and detected by an image analysis software.Matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS) and bioinformatics w... |
| |
| Keywords: | Endometrium Two dimensional gel electrophoresis MALDI-TOF-MS Proteome |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
