| 木黄酮对人胃癌细胞SGC-7901作用的研究 |
| |
| 引用本文： | 张俊文,王丕龙.木黄酮对人胃癌细胞SGC-7901作用的研究[J].胃肠病学和肝病学杂志,2004,13(2):154-157. |
| |
| 作者姓名： | 张俊文 王丕龙 |
| |
| 作者单位： | 400016,重庆,重庆医科大学附属第一医院消化内科 |
| |
| 摘 要： | 目的探讨木黄酮对体外培养的人胃癌细胞SGC-7901生长的抑制和诱导细胞凋亡的作用。方法用MTT法检测药物效应，透射电镜及流式细胞仪观察木黄酮处理SGC-7901细胞后细胞周期和细胞凋亡。细胞免疫化学观察木黄酮处理SGC-7901细胞后，SGC-7901细胞PCNA，FAS，C-mvc的变化。结果①MTT法证实木黄酮对SGC-7901细胞生长有抑制作用，并呈剂量-效应关系。②流式细胞仪分析木黄酮处理SGC-7901细胞后细胞滞留于G2／M期，并可见凋亡峰。③透射电镜可见SGC-7901细胞出现典型的细胞凋亡及坏死形态学改变。④木黄酮下调PCNA及C—myc表达，上调FAS表达。结论木黄酮对人胃癌细胞SGC-7901有抑制作用，其抑制作用可能通过下调C-mvc基因表达，上调Fas蛋白表达，下调PCNA表达，从而多途径诱导胃癌细胞凋亡和坏死、阻抑细胞周期进程，抑制SGC-7901细胞增殖。
|
| 关 键 词： | 木黄酮 胃癌 SGC-7901 癌细胞 细胞凋亡 |
| 修稿时间： | 2003年12月2日 |
The effect of Genistein induction of apoptosis on gastric cancer cell SGC-7901 |
| |
| ZHANG Junwen,WANG Pilong.The effect of Genistein induction of apoptosis on gastric cancer cell SGC-7901[J].Chinese Journal of Gastroenterology and Hepatology,2004,13(2):154-157. |
| |
| Authors: | ZHANG Junwen WANG Pilong |
| |
| Institution: | ZHANG Junwen,WANG Pilong Department of Gastroenterology,The First Affiliated Hospital,Chongqing University of Medical Science,Chongqin 400016,China |
| |
| Abstract: | Objective To study the inhibitory effects and apoptosis induction on human gastric cancer cell SGC-7901 by Genistein.Methods MTT, FCM and electron microscope were used to study the effects of Genistein on SGC-7901. Cell-immunochemistry were used to observe the change of C-myc, PCNA and Fas.Results MTT showed that the Genistein inhibited the growth of SGC-7901, and induced G 2/M cell cycle arrest, electron microscope showed that Genistein could induced cell apoptosis and necrosis, C-myc , PCNA expression were downregulated, and Fas expression were elevated.Conclusion Genistein can inhibit SGC-7901 cell growth via apoptosis induction and the arrest of cell cycle through many kinds of pathway. |
| |
| Keywords: | Genistein Gastric cancer cell SGC-7901 Cell apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
