吡格列酮对糖尿病大鼠心肌缺血再灌注损伤的影响

目的 观察糖尿病大鼠心肌缺血再灌注(MIR)时细胞间黏附分子-1(ICAM-1)和自由基代谢的变化及吡格列酮对其的影响,探讨糖尿病大鼠MIR损伤的机制.方法 制作糖尿病大鼠模型,4周后作MIR模型.糖尿病大鼠30只分为假手术对照(sham)组,MIR组和吡格列酮组.免疫组织化学法检测心肌ICAM-1蛋白表达.检测血清、心肌组织超氧化物酶(SOD)、丙二醛(MDA)、谷胱甘肽-过氧化物酶(GSH-PX)及心肌线粒体Na ,K -ATP酶、Mg2 -ATP酶、Ca2 -ATP酶活性.结果 与sham组相比,MIR组心肌线粒体Na ,K -ATP酶、Mg2 -ATP酶、Ca2 -ATP酶活性明显下降(P＜0.05),血清SOD明显降低(P＜0.01),心肌SOD和血清、心肌GSH-PX明显降低(P＜0.05),血清、心肌MDA明显升高(P＜0.05),心肌ICAM-1蛋白表达明显升高(P＜0.01).用吡格列酮4周后线粒体Na ,K -ATP酶、Mg2 -ATP酶、Ca2 -ATP酶活性高于MIR组(P＜0.05),血清、心肌SOD和GSH-PX水平高于MIR组(P＜0.05),心肌MDA低于MIR组(P＜0.01),心肌ICAM-1蛋白表达低于MIR组(P＜0.05).结论 吡格列酮可减轻糖尿病心肌缺血再灌注损伤.

Effects of pioglitazone on myocardial ischemia-reperfusion injury in diabetic rats

Objective To observe the effects of pioglitazone on the changes of the intercellular adhesion molecule-1 (ICAM-1) and free radicals in ischemia-reperfused myocardium(MIR) of diabetic rats, and investigate the reasons of myocardial ischemia-reperfusion injury in diabetic rats. Methods The diabetic rat model was established by streptozotocin injection to the abdominal cavity of Spraque-Dawlay rats. Four weeks later, the myocardial ischemic-reperfused models were established in anesthetic SD rats.Thirty SD rat...