| 抗CD_3单链抗体的构建及核酸序列分析 |
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| 引用本文： | 李冬晖,高春芳,张云,朱进.抗CD_3单链抗体的构建及核酸序列分析[J].实用医药杂志(山东),2002,19(1):37-38. |
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| 作者姓名： | 李冬晖 高春芳 张云 朱进 |
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| 作者单位： | 150医院 河南洛阳471031
(李冬晖，高春芳)，南京军区军事医学研究所 江苏南京210002
(张云)，南京军区军事医学研究所 江苏南京210002(朱进) |
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| 摘 要： | 目的探讨用抗CD3抗体重链可变区与轻链可变区基因,经重叠延伸拼接连接形成单链抗体。方法用DNA序列分析及动态模拟构象分析,并与相关软件分析,推导Scfv基因编码的氨基酸序列。结果该基因全长732bp,三维构象稳定,兼容性分数S>0。将单链抗体基因克隆于载体中,在强启动子作用下,目的蛋白的表达量约占菌体蛋白总量的20%。结论重链可变区与轻链可变区经短肽连接而成的单链抗体,具有分子量小,免疫原性低的特性,具有较广的应用前景。
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| 关 键 词： | 单链抗体 核酸分析 原核表达 |
| 修稿时间： | 2001年3月5日 |
The construction of anti-CD3 single chain Fv and DNA sequence analysis |
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| Li Donghui,GaoChunfang,Zhang Yun,et al..The construction of anti-CD3 single chain Fv and DNA sequence analysis[J].Practical Journal of Medicine & Pharmacy,2002,19(1):37-38. |
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| Authors: | Li Donghui GaoChunfang Zhang Yun |
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| Institution: | Li Donghui,GaoChunfang,Zhang Yun,et al.The150th Central Hospital of PLA,Louyang471031,China |
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| Abstract: | Objective To investigate single chain antibody linked by the variable regions genes of heavy chain(VH)and light chain of anti-CD 3 antibody by means of splicing,overlapping and exten sion(SOE).Methods The DNA sequence of the anti-CD 3 single chain Fv was analysed by DNA se quence analysis and dynamic simulation conformation with relative software,derivating the amino-acid se quences coded by ScFv gene.Results The anti-CD 3 single chain Fv(ScFv)gene has732bp,3D-conformation stabi lization,com patibility S>0.The expression level of aim protein cod ed by the gene is about 20%of total bacte ria pro tein level.Con clusion anti-CD 3 ScFv-coding protein has small molecule weight ,low im munogenic ity,thus has wider prospect of application. |
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| Keywords: | Single chain Fv DNA sequence analysis Prokaryotic expression |
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