| 葡萄糖-6-磷酸酶在大白鼠心肌细胞超微结构的定位 |
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| 引用本文： | 韩玉升,祁小平,陈玉英. 葡萄糖-6-磷酸酶在大白鼠心肌细胞超微结构的定位[J]. 解剖学报, 1987, 0(4) |
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| 作者姓名： | 韩玉升 祁小平 陈玉英 |
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| 作者单位： | 上海第二医科大学生物物理教研室，上海第二医科大学生物物理教研室，上海第二医科大学生物物理教研室 |
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| 摘 要： | 葡萄糖-6-磷酸酶(G6Pase)是内质网的标志酶,本研究观察了大白鼠左心室心肌细胞的G6Pase的定位。实验结果表明,G6Pase活性遍布于心肌细胞的肌浆网(内质网)腔内,包括肌原纤维之间的肌浆网小管、肌膜下池和核膜。尤其是酶的反应产物在肌原纤维处显示了肌浆网小管的特征性分布:即在A带处肌浆网小管排列紧密形成致密网格,而在I带处排列稀疏,形成多角形大网格。这种排列特点可能和肌肉的舒缩功能有关,在收缩时I带的细丝向A带粗丝处滑动,肌节缩短。在I带处肌浆网小管排列成大网格、稀疏,便于伸长或缩短,是适应性的分布。
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| 关 键 词： | 超微结构 心肌细胞 肌浆网 葡萄糖-6-磷酸酶 大鼠 |
ULTRASTRUCTURAL LOCALIZATION OF G6PASE ACTIVITY IN THE RAT MYOCARDIAL CELLS |
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| Han Yusheng,Qi Xiaoping,Chen Yuying. ULTRASTRUCTURAL LOCALIZATION OF G6PASE ACTIVITY IN THE RAT MYOCARDIAL CELLS[J]. Acta Anatomica Sinica, 1987, 0(4) |
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| Authors: | Han Yusheng Qi Xiaoping Chen Yuying |
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| Abstract: | Glucose-6-Phosphatase (G6Pase) was regarded as a marker enzyme of the endoplasmic reticulum in a number of different cells. The purpose of this report is to study the localization of G6Pase activity in the rat left ventricular myocardial cells. G6Pase activity was found in the lumen of the nuclear envelope, the sarcoplasmic reticulum(SR) and the subsarcolemmal cisterns. The SR tubules between the adjacent myofibrils displayed characteristic distribution on their longitudinal profiles, as a curtain-like network, the tubules appeared to be tight network facing A-band, whereas tubules formed large polygonal meshes facing I-band. It is thought that the SR tubules facing A- and I-bands, respectively, represented an adaptation of SR to the selective shortening of the myofibrils at the I-band during contraction. |
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| Keywords: | Ultrastructure Myocardial Cells Sarcoplasmic reticulum G6Pase Rat |
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