膀胱肿瘤术后化疗的个体化研究

目的：探讨膀胱肿瘤体外药物敏感试验的方法,并观察其指导临床用药的效果.方法：38例膀胱肿瘤患者作为药敏组,对其肿瘤标本在不同药物及同一药物不同浓度中进行体外细胞培养,应用单四唑（MTT）法检测其生长抑制率,并以此指导临床用药及术后化疗.另以30例常规应用羟喜树碱治疗的患者为对照组,分别观察两组膀胱肿瘤的年复发次数.结果：药物对肿瘤细胞的抑制率与药物的浓度呈正相关;联合用药对肿瘤细胞的抑制率明显高于单一用药.临床观察显示,药敏组肿瘤年复发次数明显低于对照组.结论：应用MTT法检测体外培养的肿瘤细胞生长抑制率是指导膀胱癌术后灌注化疗药物选择的有效方法.

The study on respective chemotherapy of bladder tumors

Objective: To provide a scientific method for the reasonable use of the drugs,and to use it as guidelines for clinical chemotherapy. Methods: Samples of test group were obtained from 38 cases of bladder cancer.which were cultivated with different medicines and then examined the inhibiting rate with MTT.The medicine with the highest inhibiting rate was selected to treat bladder cancer.30 cases of bladder cancer were treated with HCPT routinely as control group.The rate of tumor recurrence between two groups was compared. Results:The inhibiting rate on tumour cells was positively related to the concentration of drugs,the combined chemotherapy was superior to single drug.Statistic results showed significant difference between the test group and control group(P<0.01). Conclusion: The drug sensitivity test of the original passage cell culture in vitro of bladder cancer provides a good guideline for the patients of postoperative intravesical chemotherapy.The method of MTT is fast and convenient.