Hepatitis B virus pre S1 deletion is related to viral replication increase and disease progression |
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Authors: | Seoung-Ae Lee Ki-Jeong Kim Hong Kim Won-Hyuk Choi Yu-Sub Won Bum-Joon Kim |
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Institution: | Seoung-Ae Lee, Hong Kim, Bum-Joon Kim, You-Sub Won, Department of Biomedical Sciences, Microbiology and Immunology, and Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, South KoreaKi-Jeong Kim, Department of Microbiology, School of Medicine, Chung-Ang University, Seoul 110-799, South KoreaWon-Hyuk Choi, Department of Internal Medicine, Konkuk University, Seoul 110-799, South Korea |
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Abstract: | AIM: To investigate the clinical implications of hepatitis B virus (HBV) preS1 deletion.METHODS: We developed a fluorescence resonance energy transfer-based real-time polymerase chain reaction (RT-PCR) that can detect four genotypes (wild type, 15-bp, 18-bp and 21-bp deletion). The PCR method was used in two cohorts of Korean chronic HBV subjects with genotype C infections. Cohort?I??included 292 chronic HBV subjects randomly selected from Cheju National University Hospital (Jeju, South Korea) or Seoul National University Hospital (Seoul, South Korea), and cohort II included 90 consecutive chronic HBV carriers recruited from Konkuk University Hospital (Seoul, South Korea); the cohort II patients did not have hepatocellular carcinoma or liver cirrhosis.RESULTS: The method proposed in this study identified 341 of 382 samples (89.3%). Deletion variants were identified in 100 (29.3%) of the 341 detected samples. In both cohorts, the subjects with deletions had a significantly higher Hepatitis B virus e antigen (HBeAg)-positive seroprevalence cohort?I, wild (51.0%) vs deletion (75.0%), P < 0.001; cohort II, wild (69.2%) vs deletion (92.9%), P = 0.002] and higher HBV DNA levels cohort?I, wild (797.7 pg/mL) vs deletion (1678.9 pg/mL), P = 0.013; cohort II, wild (8.3 × 108 copies/mL) vs deletion (2.2 × 109 copies/mL), P = 0.049], compared to subjects with wild type HBV.CONCLUSION: HBV genotype C preS1 deletion may affect disease progression in chronic HBV subjects through an extended duration of HBeAg seropositive status and increased HBV replications. |
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Keywords: | Hepatitis B virus PreS1 start codon deletion Hepatitis B virus e antigen Hepatocellular carcinoma Genotype C |
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