Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon |
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| Authors: | Masayoshi Fukuda Tomohiro Mizutani Wakana Mochizuki Taichi Matsumoto Kengo Nozaki Yuriko Sakamaki Shizuko Ichinose Yukinori Okada Toshihiro Tanaka Mamoru Watanabe Tetsuya Nakamura |
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| Affiliation: | 1.Department of Gastroenterology and Hepatology.;2.Research Center for Medical and Dental Sciences.;3.Department of Human Genetics and Disease Diversity.;4.Bioresource Research Center.;5.Department of Advanced Therapeutics for GI Diseases, Tokyo Medical and Dental University, 113-8519 Tokyo, Japan |
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| Abstract: | To develop stem cell therapy for small intestinal (SI) diseases, it is essential to determine whether SI stem cells in culture retain their tissue regeneration capabilities. By using a heterotopic transplantation approach, we show that cultured murine SI epithelial organoids are able to reconstitute self-renewing epithelia in the colon. When stably integrated, the SI-derived grafts show many features unique only to the SI but distinct from the colonic epithelium. Our study provides evidence that cultured adult SI stem cells could be a source for cell therapy of intestinal diseases, maintaining their identity along the gastrointestinal tract through an epithelium-intrinsic mechanism. |
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| Keywords: | small intestinal stem cell transplantation stem cell niche Paneth cell regenerative medicine |
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