| 人大肠癌细胞蛋白质组中免疫球蛋白重链可变区和B7-1的表达 |
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| 引用本文： | Zhang YT,Geng YP,Lai BC,Si LS,Wang YL. 人大肠癌细胞蛋白质组中免疫球蛋白重链可变区和B7-1的表达[J]. 中华肿瘤杂志, 2005, 27(11): 648-652 |
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| 作者姓名： | Zhang YT Geng YP Lai BC Si LS Wang YL |
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| 作者单位： | 1. 710061,西安交通大学医学院第一附属医院消化内科
2. 西安交通大学生命科学与技术学院癌症研究所生物医学信息工程教育部重点实验室 |
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| 基金项目： | 西安交通大学自然科学基金资助项目（Y100-573006）;西安交通大学博士论文基金资助项目（DFXJTU2002-11） |
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| 摘 要： | 目的 分析人大肠癌LS174T及SW480细胞株的蛋白质表达谱。方法 通过固相pH梯度双向凝胶电泳分离人大肠癌LS174T及SW480细胞总蛋白质,经基质辅助激光解吸电离-飞行时间质谱（MALDI-TOF-MS）获得肽质量指纹图（PMFs）,通过Mascot软件查询蛋白质数据库进行蛋白质的初步鉴定。结果 获得了分辨率较高的人大肠癌LS174T及SW480细胞的双向电泳图谱,初步鉴定了一批蛋白质,包括免疫球蛋白重链可变区（IgVH）和共刺激分子B7-1,并通过电喷雾离子阱串联质谱（ESI-MS／MS）分析和免疫细胞化学染色得到进一步证实。结论 在人大肠癌LS174T和SW480细胞中存在IgVH和B7-1的表达,这一发现有助于进一步阐明肿瘤的免疫逃逸机制。
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| 关 键 词： | 大肠癌 蛋白质组 免疫球蛋白重链可变区 B7-1 |
| 收稿时间： | 2004-04-19 |
| 修稿时间： | 2004-04-19 |
Expression of IgVH and B7-1 in proteome of the human colorectal carcinoma cell lines |
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| Zhang Ying-tao,Geng Yi-ping,Lai Bao-chang,Si Lü-sheng,Wang Yi-li. Expression of IgVH and B7-1 in proteome of the human colorectal carcinoma cell lines[J]. Chinese Journal of Oncology, 2005, 27(11): 648-652 |
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| Authors: | Zhang Ying-tao Geng Yi-ping Lai Bao-chang Si Lü-sheng Wang Yi-li |
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| Affiliation: | The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Cancer Research, School of Life Science & Technology, Xi'an Jiaotong University, Xi'an 710061, China. |
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| Abstract: | Objective To conduct a proteomic analysis of human colorectal carcinoma cell lines LS174T and SW480 by two-dimensional electrophoresis(2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS).Methods The total proteins of human colorectal carcinoma cell lines LS174T and SW480 were separated with 2-DE using immobilized pH gradient strips and analyzed by MALDI-TOF-MS to obtain peptide mass fingerprints(PMFs).Proteins were identified by using Mascot software to search protein databases.Results Good resolution 2-DE maps were obtained.Some proteins including immunoglobulin heavy chain variable region(IgVH) and co-stimulatory molecule B7-1 were identified.IgVH and B7-1 were confirmed by electrospray ionization tandem spectrometry(ESI-MS/MS) and immunocytochemistry.Conclusion There are IgVH and B7-1 expressions in human colorectal carcinoma cell lines LS174T and SW480.Results obtained will help to elucidate the mechanisms of tumor immune escape. |
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| Keywords: | Colorectal carcinoma Proteome IgVH B7-1 |
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