| HPLC法测定单叶细辛中马兜铃酸A的含量 |
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| 引用本文： | 刘晋仙,印成霞. HPLC法测定单叶细辛中马兜铃酸A的含量[J]. 齐鲁药事, 2010, 29(10): 593-595 |
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| 作者姓名： | 刘晋仙 印成霞 |
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| 作者单位： | 东营市药品检验所,山东,东营,257091 |
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| 摘 要： | 目的 HPLC法测定单叶细辛中马兜铃酸A的含量。方法采用Sunfire C18柱(150mm×4.6mm,5μm);流动相:甲醇-水-醋酸(60∶36∶4);检测波长316nm。结果线性范围为6.60~39.60μg.mL-1(r=0.9995),平均回收率为95.8%,RSD为0.8%(n=6)。结论该方法简便、准确,可用于单叶细辛中马兜铃酸A的质量控制。
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| 关 键 词： | 单叶细辛 马兜铃酸A 高效液相色谱法 |
Determination of aristolochic acid A in Asarum himalaicum by HPLC |
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| LIU Jin-xian,YIN Cheng-xia. Determination of aristolochic acid A in Asarum himalaicum by HPLC[J]. qilu pharmaceutical affairs, 2010, 29(10): 593-595 |
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| Authors: | LIU Jin-xian YIN Cheng-xia |
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| Affiliation: | (Dongying Institute for Drug Control,Dongying 257091) |
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| Abstract: | OBJECTIVE To establish a method for determination of aristolochic acid A in Asarum himalaicum.METHODS The conditions of HPLC were Sunfire C18 column（150mm×4.6mm,5μm）,methanol-water-acetic（60∶36∶4） as mobile phase.The detection wavelength was 316nm.RESULTS The linear range was 6.60～39.60μg·mL^-1（r=0.9995）,the average recovery was 95.8%,and the RSD was 0.8%（n=6）.CONCLUSION The medthod was simple,accurate and can be used to control quality of aristolochic acid A in Asarum himalaicum. |
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| Keywords: | Asarum himalaicum Aristolochic acid A HPLC |
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