川芎嗪注射液促心梗后大鼠缺血心肌血管新生作用及对相关生长因子影响的研究

目的:观察川芎嗪注射液对心肌梗死(MI)后大鼠缺血心肌血管新生作用,并探讨其机制。方法:雄性Wist-ar大鼠,结扎左冠状动脉造成急性心肌梗死(AMl)模型,随机分为川芎嗪注射液组、麝香保心丸组(阳性对照)、模型组(阴性对照),并设假手术组。6周后处死动物,用免疫组织化学法,检测各组大鼠缺血心肌中微血管数(MVC)、微血管密度(MVD)及血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)、血小板衍生生长因子β(PDGF-β)、胰岛素样生长因子(IGF-1)蛋白的表达,用病理图像分析系统测定生长因子蛋白表达灰度值并进行半定量分析。结果:模型组、麝香保心丸组及川芎嗪组大鼠MI边缘区MVC和MVD较假手术组明显增多(P<0.05);川芎嗪组大鼠缺血心肌MVC和MVD较模刑组明显增加(P<0.05),与麝香保心丸组作用相似(P>0.05)。模型组VEGF、bFGF、PDGF-β、IGF-1蛋白表达及其灰度值明显高于假手术组(P<0.05);川芎嗪组四种生长因子蛋白表达灰度值明显高于模刑组(P<0.05),但低于麝香保心丸组,bFGF、PDGF-β、IGF-1蛋白表达灰度值两组相比无统计学差异(P>0.05),VEGF蛋白表达灰度值两组相比有统计学差异(P<0.05)。结论:川芎嗪注射液有促MI后大鼠缺血心肌血管新生的作用,其机制可能与促进VEGF、bFGF、PDGF-β、IGF-1的表达有关。

Study of Angiogenesis and Correlative Growth Factor Effects of Tetra Methyl Pyrazine Injection on the Ischemic Myocardium of the Rats after Myocardial Infarction

Objective:To study the angiogenesic effects and molecular mechanisms of tetra methyl pyrazine injection on the ischemic myocardium of the rats after myocardial infarction(MI).Methods:Male Wistar rats were ligated left coronary artery to be made into models of acute myocardial infarction(AMI).All rats were randomly divided into tetra methyl pyrazine injection,Shexiang baoxin pills(positive control group)model(negative control group)group and sham operation group.Treated them respectively for six weeks.Microvasscular vascular count(MVC),Microvasscular vascular density(MVD),Vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),insulin-kind growth factor(IGF)and Platelet-derived endothelial growth factor(PDGF)inischemic myocardium of MI rats were detected by immunobistochemistry assay;The values of protein expression of VEGF,bFGF,PDOF-β and IGF-1 on ischemic myocardium were calculated and analied by image analysis systerm.Results:There were more MVC and MVD in ischemic myocardium of AMI rats of modle group,tetra methyl pyrazine injection group and Shexiang baoxin pills group than sham operation group.MVC and MVD were higher in ischemic myocardium of AMI rats of tetra methyl pyrazine injection group than those of modle group.Compared with Shexiang baoxin pills,they have the same roll.The values of protein expression of VEGF,bFGF,PDGF-β and IGF-1 in modle group were significantly elevated compared with that in sham operation group.In tetra methyl pyrazine injection group,they were significantly elevated compared with that in modle group.The values of protein expression of VEGF,bFGF,PDGF-β and IGF-1 in tetra methyl pyrazine injection group were lower compared with that in Shexiang baoxin pills group.There were not significantly statistics differences in the expression of protein of bFGF,PDGF-β,IGF-1 in tetra methyl pyrazine injection group compared with that in Shexiang baoxin pills group.But there was significantly statistics difference in the expression of protein of VEGF in tetra methyl pyrazine injection group compared with that in Shexiang baoxin pills group.Conclusions:Tetra methyl pyrazine injection has the role of promoting angiogenesis of the ischemic myocardium in rats after MI.Its mechanisms can be related with stimulating the expression of vescular growth factors.