ELISA测定Alzheimer病脑脊液磷酸化神经丝和双螺旋丝抗原活性

应用160～200KD磷酸化神经丝(PNF)和双螺旋丝(PHF)单克隆抗体建立竞争抑制ELISA。并检测了21例Alzheimer病(AD)、17例多发梗塞性痴呆、33例其他神经疾病、33例精神分裂症及40例正常人的CSF中PNF和PHF抗原活性。结果AD组CSF中PHF抗原活性明显高于其他各组;而其PNF/PHF比值明显低于其他各组。本法有可能成为诊断AD的实验室方法。

Detection of Phosphorylated Neurofilament and Paired Helical Filament Immunoreactivities in Alzheimer Cerebrospinal Fluid by ELISA

With the monoclonal antibody of phosphorylated neurofilaments (PNF) (molecular weight 160-200KD) and paired helical filaments (PHF) respectively, a competitive enzyme-linked immunosorbent assay (ELISA) has been established for PNF and PHF immunoreac-tivities in the cerebrospinal fluid (CSF). Using the assay, we measured PNF and PHF im-munoreactivities in CSF from 21 patients with Alzheimer's disease (AD), 17 with multi-in-farct dementia, 33 with other neurological diseases control, 33 patients with Schizophrenia, and 40 normal control. It was found that the levels of PHF immunoreactivity measured in CSF were significantly higher in AD than in control groups (P<0. 05). The values of PNF/ PHF ratio in CSF were significantly lower in the AD than in the control groups (P<0. 001). These findings demonstrate that this assay is specific and sensitive, and might be developed to be significant by the means of clinical diagnosis of the AD.