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| 苦参碱对P物质诱导角质形成细胞和真皮成纤维细胞趋化蛋白-1表达的影响 | |
| 引用本文： | 蒲江,孙小萌,杨帝顺,刘继勇,余克强.苦参碱对P物质诱导角质形成细胞和真皮成纤维细胞趋化蛋白-1表达的影响[J].中国中西医结合皮肤性病学杂志,2010,9(4):201-205. |
| 作者姓名： | 蒲江 孙小萌 杨帝顺 刘继勇 余克强 |
| 作者单位： | 1. 上海长海医院,上海,200433 2. 武警总医院,北京,100039 3. 南方医科大学,广州,510515 |
| 基金项目： | 上海市科委中药现代化专项基金 |
| 摘 要： | 目的研究苦参碱对P物质诱导皮肤表皮角质形成细胞系HaCaT细胞和真皮成纤维细胞表达单核细胞趋化蛋白-1(MCP-1)的影响。方法采用MTT法考察苦参碱对两种皮肤细胞生存性的影响;ELISA方法测定P物质诱导两种皮肤细胞分泌MCP-1的时效关系与量效关系。以P物质刺激HaCaT细胞和真皮成纤维细胞,加入不同剂量的苦参碱共孵育24h,测定苦参碱对MCP-1表达的影响。结果以10-8mol/LP物质刺激HaCaT细胞和真皮成纤维细胞24h可以显著增加两种皮肤细胞MCP-1的分泌量,不同浓度的苦参碱均可以抑制皮肤细胞MCP-1的分泌。结论苦参碱可能通过抑制趋化因子MCP-1的表达而发挥抗皮肤炎症作用。 |
| 关 键 词： | 苦参碱 P物质 皮肤炎症 单核细胞趋化蛋白-1 |
Effect of Matrine on the Expression of Moncyte Chemoattractant Protein-1 Induced by Substance P in HaCaT Cells and Fibroblasts |
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| PU Jiang,SUN Xiao-meng,YANG Di-shun,LIU Ji-yong,YU Ke-qiang.Effect of Matrine on the Expression of Moncyte Chemoattractant Protein-1 Induced by Substance P in HaCaT Cells and Fibroblasts[J].Chinese J of Dermatovenerology Integr Tradit and West Med,2010,9(4):201-205. | |
| Authors: | PU Jiang SUN Xiao-meng YANG Di-shun LIU Ji-yong YU Ke-qiang |
| Institution: | PU Jiang1,SUN Xiao-meng 2,YANG Di-shun 1,LIU Ji-yong 1,YU Ke-qiang3 1. Shanghai Changhai Hospitial,Shanghai 200433,China,2. General Hospital of Armed Police Force,Beijing 100039,3. SouthernMedicalUniversity,Guangzhou510515 |
| Abstract: | Objective To study the effect of Matrine on the expression of moncyte chemoattractant protein-1(MCP-1) induced by substance P(SP)in keratinocytes line HaCaT cells and fibroblasts. Methods The time and dose-response relationship of MCP-1 release in HaCaT cells and fibrobalsts stimulated by SP were determinated by ELISA method. The effect of Matrine on the release of MCP-1 in HaCaT cells and fibrobalsts was measured by ELISA method. Results The expression of MCP-1 in HaCaT cells and fibrobalsts stimulated by ... |
| Keywords: | Matrine substance P cutaneous inflammation moncyte chemcattractant protein-1 |
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