HPLC法测定人血浆中头孢羟氨苄含量

目的：建立用内标法测定人血浆中头孢羟氨苄浓度的HPLC方法。方法：用c（高氯酸）=10％直接沉淀血浆蛋白。以对氨基苯甲酸为内标，测定血浆中头孢羟氨苄的浓度。结果：血浆中杂质不干扰样品峰和内标峰，20．00、2．00、0．20μg／mL的回收率分别为98．10％、98．70％、91．10％；批内精密度高、中、低浓度分别为3．80％，5．60％，7．30％;批间精密度高、中、低浓度分别为4．10％，6．50％，9．70％，最低检测限为0．10μg／mL，线性范围为0．20～30．00μg／mL。结论：本法稳定、精密度好，准确可靠，适用于头孢羟氨苄血浆浓度测定。

Determination of cefadroxil in the human blood plasma by HPLC

Objective:To establish the determination method of cefadroxil in healthy volunteers plasma.Methods: Plasma protein was precipitated with perchloric acid(10.00%),and para-amino benzoic acid was used as internal standard. Plasma concentrations of cefadroxil and internal standard were determined by HPLC-UV.Results:The chromatographic peak of cefadroxil and internal standard was not interfered by foreign matter.Recovery rate of 20.00、2.00,0.20μg/mL was 98.10%,98.70%,91.10%,respectively.Precision in or between batches was less than 8.00% and 10.00%,respectively. The lowest detectable limit was 0.10μg/mL and the linear range was 0.20--30.00 ~tg/mL.The freeze thawing of plasma was stable.Conclusion:The method was reliable and stable and it was suitable for determination the concentration of cefadroxil in plasma.