严重急性呼吸综合征患者核壳蛋白和刺突蛋白S1区的特异性抗体研究

目的研究严重急性呼吸综合征(SARS)患者血清中抗SAPS冠状病毒(SAPS-CoV)核壳蛋白(N)及刺突蛋白(S)S1区特异性抗体的产生规律,评价这两种蛋白在SAPS诊断研究中的作用。方法采用ELISA法检测86例确诊SAPS患者血清中针对N和S1蛋白IgG(N-IgG和S1一IgG),并与SAPS-CoV IgG水平进行比较。结果两种蛋白的特异性抗体的阳性率均随着病程的延长而增高。N-IgG在发病第1周检出阳性率为14％(6／44),第2周为56％(10／18),第3周达到100％(24／24);S1-IgG第1周检出阳性率为5％(2／44),第2周为39％(7／18),第3周阳性率达到83％(20／24)。两种蛋白抗体的检测结果分别与SAPS-CoV IgG结果比较,检测符合率分别为88％(76／86)和83％(71／86)。结合免疫印迹和ELISA两种方法检出正常人群SAPS-CoV N-IgG阳性率为1．88％(14／745)。结论SAPS-CoV N和S1重组蛋白对于SAPS诊断试剂的研究具有重要价值。

Specific antibodies against the nucleocapsid protein and S1 domain of spike glycoprotein in patients with severe acute respiratory syndrome

Objective To explore the laws of the appearance of the specific serum antibodies against the nucleocapsid (N) protein and the S1 domain of spike (S) glycoprotein in patients with severe acute respiratory syndrome (SARS) and to evaluate the value of these two proteins to be used as diagnostic makers for SARS. Methods The serum samples of 86 patients with SARS confirmed clinically and serologically, 31 males and 55 females, aged 9~86, with the course of disease of 1~81 days, and 745 healthy persons were collected during the course. The specific immunoglobulin G (IgG) against N protein, IgG against S1 domain of S protein, and the SARS-CoV IgG in these sera were detected by ELISA. Results The positive rates of anti-N-IgG, anti S1-IgG, and anti-SARS-CoV IgG were 14% (6/44), 5% (2/44), and 14% (6/44) respectively in the first week of the course of disease, 56%(10/18), 39%(7/18), and 56%(10/18)respectively in the second week, and 100% (24/24), S1-IgG 83%(20/24), and 100% (24/24) respectively in the third week. The agreement rates of the results of anti-N-IgG and anti-s1-IgG with that of the anti-SARS-CoV IgG were 88%(76/86) and 83%(71/86) respectively. The anti-SARS-CoV IgG positive rate in the healthy persons was 1.88% (14.745). Conclusion The N protein and S1 protein may be used as antigens in the development of serological assay for detection of SARS-CoV infection.