乙型肝炎病毒基因疫苗诱导小鼠产生特异免疫应答

构建编码乙型肝炎病毒（ＨＢＶ）表面蛋白Ｓ的重组质粒ｐＣＲ３．１－Ｓ。将之直接肌肉注射Ｂａｌｂ／ｃ小鼠，观察小鼠ＨＢＶ特异的免疫应答。以ＥＬＩＳＡ法检测小鼠血清，＾３Ｈ－ＴｄＲ掺入法测定淋巴细胞增殖，＾５１Ｃｒ４ｈ释放法检测淋巴细胞杀伤功能。结果表明，与空载体对照组相比较，基因疫苗诱发小鼠产生良了的抗ＨＢｓ反应及ＨＢＶ特异的细胞免疫应答（Ｐ〈０．０５），提示基因疫苗ｐＣＲ３．１－Ｓ有可能成为控制ＨＢ

Gene vaccines of hepatitis B virus induces specific immune responses in mice

Recombinant eukaryotic expression plasmids of PCR3. 1-S encoding S protein of hepatitis B virus(HBV)were constructed as gene vaccines. Balb/c mice were inoculated with these plasmids so that immune responses to HBV could be studied. The titers ot antibodies to HBV major proteins were measured by ELISA and specific cellular responses including lyrnphocyte proliferation and cytotoxic T lymphocyte activity were detected through 3H-TdR incorporation and 4-hour 51Cr release assays. It turns out that mice immunized with gene vaccines have higher antiHBs and specific cellular responses to HBV than ones with vector plasmids. These results reveal that gene vaccine of PCR3. l-S could be another weapon controlling HBV infection.