厚朴的任意引物PCR指纹图谱分析

目的：鉴别中药材厚朴，凹叶厚朴及其常见的伪品和混淆品，方法：采用任意引物PCR（arbitrarily primed PCR,AP-PCR)技术扩增植物基因组DNA样品。结果：AP－PCR技术获得清晰可靠的DNA指纹图谱，根据琼脂糖凝胶上显示的DNA带型差异可迅捷地区分厚朴，凹叶厚朴及其伪品，混淆品。结论：为应用AP－PCR技术在分子水平上鉴别中药材厚朴，保证引种的准确性奠定了基础。

Fingerprint of arbitrarily primed polymerase chain reaction (AP-PCR) for Magnolia officinalis

Object To identify Magnolia officinalis Rehd et Wils and M biloba Rehd et Wils as well as their counterfeits and easily confusable substitutes Methods Total genomic DNA samples of ten plant species were amplified by arbitrarily primed PCR (AP-PCR) Results Ten samples were distinguished through their amplified DNA banding patterns on the agarose gels after eletrophoresis, so as to differentiate the counterfeits and easily confusable substitutes of M officinalis and M biloba. Conclusion AP-PCR is useful for identifying M officinalis at molecular level, as well as valuable to guarantee introducing original plant correctly