| 人原发性肝细胞癌中HBxAg的免疫组化及免疫电镜研究 |
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| 引用本文： | 徐富,王文亮,隋延仿,杨萍,王冰枫. 人原发性肝细胞癌中HBxAg的免疫组化及免疫电镜研究[J]. 医学争鸣, 1993, 0(6) |
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| 作者姓名： | 徐富 王文亮 隋延仿 杨萍 王冰枫 |
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| 作者单位： | 第四军医大学病理学教研室(徐富，王文亮，隋延仿，杨萍)，第四军医大学病理学教研室(王冰枫) |
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| 摘 要： | 作者用免疫组化和胶体金免疫电镜方法,观察乙型肝炎x抗原(HBxAg)在肝癌组织(35例)及癌旁肝组织(29例)内的分布及亚细胞定位。结果显示,HBxAg在癌组织及癌旁肝组织内的检出率分别为60.0％和82.8％,阳性物质主要位于细胞浆内。电镜观察,HBxAg主要定位于粗面内质网和胞浆基质中,少数位于核内,并可见游离核糖体及核孔附近阳性,推测HBxAg在内质网及游离核糖体上合成,并可经核孔进入核内。
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| 关 键 词： | 肝细胞癌 乙型肝炎x抗原 免疫组织化学 显微镜检查 电子 免疫 |
Immunohistochemical and immunoelectron microscopic study of hepatitis B virus X antigen in human primary hepatocellular carcinoma |
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| XU Fu,WANG Wen-Liang,SUI Yan-Fang,YANG Ping and WANG Bing-Feng. Immunohistochemical and immunoelectron microscopic study of hepatitis B virus X antigen in human primary hepatocellular carcinoma[J]. Negative, 1993, 0(6) |
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| Authors: | XU Fu WANG Wen-Liang SUI Yan-Fang YANG Ping WANG Bing-Feng |
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| Affiliation: | XU Fu,WANG Wen-Liang,SUI Yan-Fang,YANG Ping and WANG Bing-Feng Department of Pathology |
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| Abstract: | HBxAg in 35 cases of HCC and 29 of surrounding nontumor hepatic tissues was detected by im-munohistochemistry and immunoelectron microscopy. The results showed that HBxAg-positive was of 21 cases (60. 0%) in cancerous tissues and 24 (82. 8%) in nontumor tissues with immunohistochemical staining. HBxAg-positive substance was mainly localized in the cytoplasm. Under immunoelectron microscopy, HBxAg-positive substance was mainly in rough endoplasmic reticulum (RER) and cytoplasmic matrix, a few of cases were in the cellular nuclei. In addition, HBxAg-positive substance was also observed on the free ribo-some and near the nuclear pore. It suggested that the HBxAg may be synthesized on the ribosorne and RER, and could enter nuclei through nuclear pore. |
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| Keywords: | hepatocellular carcinoma hepatitis B x antigen immunohistochemistry microscopy electron immunological |
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