血小板冷冻保存的研究

用终浓度4％二甲基亚砜(DMSO)作为防冻剂,于—30℃和—80℃冷冻保存血小板以开展低温保存血小板的输注。体内外研究结果表明:DMSO对血小板粘附和单一诱聚剂所致的聚集有可逆性抑制作用;洗涤去除DMSO后,粘附、聚集可以恢复。联合诱聚剂所致的聚集不受DMSO影响。冻存后的血小板粘附、聚集和低渗休克反应较冻前有所降低,与保存时间(1和3个月)无关。—80℃保存的血小板质量优于—30℃保存者。输注—80℃保存的血小板可提高患者的血小板数,因而能有效地预防和控制因血小板减少导致的出血。

Study of Cryopreservation of Human Platelets

Using the dimethylsulfoxide (DMSO)with final concentration of 4% as cryoproteclant, the human platelets were stored at - 30℃ and - 80℃ to provide cryopreserved platelets transfusion service. The results of in vivo and in vitro studies showed that DMSO could reversibly inhibit the platelet adhesion and its aggregation induced by the single aggregating agent. The platelet abhe-sion and aggregation could be recovered after washing and removing DMSO. The platelet aggregation caused by the combined aggregating agents wasn't influenced by DMSO. The adhesion, aggregation and resulting hypotonic shock reaction of cryopreserved platelets were used to be lower than those of fresh platelets, independent on the storage timed or 3 months). The quality of the platelets stored at - 80℃ was better than that at - 30℃. Being transfused with the platelets stored at - 80# could raise the platelet counts in patients, and effectively prevent and control the haemorrhage caused by thrombocytopenia.