非小细胞肺癌患者血清Ras相关区域家族1A基因异常甲基化检测及意义

目的 探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)患者血清中Ras相关区域家族1A(Ras association domain family 1A,RASSF1A)基因启动子区域甲基化状况及其临床意义.方法 基因测序法检测62例NSCLC患者、30例肺部良性疾病患者和16名健康体检者血清中RASSF1A启动子区域甲基化状况,并分析其与临床特征的关系.结果 RASSF1A甲基化检出率在NSCLC患者为45.16%(28/62),而肺部良性疾病患者和健康体检者血清未检出(P<0.05).NSCLC患者血清RASSF1A基因甲基化率在Ⅲ～Ⅳ期高于Ⅰ～Ⅱ期,低、未分化组高于高、中分化组(P <0.05),与年龄、性别、吸烟指数、病理类型无关.结论 RASSF1A异常甲基化可能在NSCLC发生、发展中起重要作用,有望成为NSCLC辅助诊断和预后判断的分子标记.

Detection and significance of abnormal methylation of Ras association domain family 1A gene in serum of non-small cell lung cancer patients

Objective To investigate the promoter region methylation of Ras association domain family 1A (RASSF1A) gene in serum of non-small cell lung cancer (NSCLC) patients and its clinical significance.Methods The promoter region methylation of RASSF1A in the serum of 62 patients with NSCLC,30 patients with benign pulmonary disease and 16 health subjects was detected by gene sequencing,and its relationship with clinical features was analyzed.Results The detection rate of RASSF1A methylation was 45.16 % (28/62) in NSCLC patients,and RASSF1A methylation was not detected in the serum of patients with benign pulmonary disease and health subjects ( P <0.05).In NSCLC patients,the methylation rate of RASSF1A gene in stage Ⅲ-Ⅳ was higher than that in stage Ⅰ-Ⅱ,the methylation rate of RASSF1A gene in poorly differentiated and undifferentiated group was higher than that in highly and moderately differentiated group ( P < 0.05),and there was no correlation between methylation rate of RASSF1A gene and age,sex,smoking index,pathological type.Conclusions Abnormal methylation of RASSF1 A may play an important role in the development of NSCLC,and it is hopeful to become a biomarker for diagnosis and prognosis of NSCLC.