块花柳珊瑚化学成分的研究

目的对采自中国广西省北海附近的块花柳珊瑚(Anthogorgia sp.)进行化学成分的研究。方法用硅胶柱层析(石油醚:丙酮=99:1,49:1,39:1,34:1,29:1,24:1,19:1,14:1,11:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1:1,100%丙酮;氯仿:甲醇=24:1,19:1,14:1,9:1,7:1,5:1,3:1,1:1,1:4,1:9,1:39,100%甲醇)梯度洗脱和SephadexLH-20凝胶柱层析(正己烷:氯仿:甲醇2:1:1洗脱)对块花柳珊瑚丙酮提取物的乙醚部分进行分离纯化,并利用经1HNMR、13CNMR、MS等现代光谱技术对其进行结构鉴定。结果分离得到6个甾醇类化合物以及1个神经酰胺,其结构分别为:麦角甾-5,24(28)-二烯-3β-醇(1)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(2)、(22E,24S)-麦角甾-5,22-二烯-3β-醇(3)、胆甾-5-烯-3β,7β,19α-三醇(4)、(22E)-胆甾-5,22-二烯-3β-醇(5)、胆甾醇(6)、N-正十六碳酰基-正十八碳-4(E)-烯鞘胺醇(7)。结论这些化合物均首次从该种海洋动物中分离得到。

Studies on chemical constituents of Anthogorgia sp.

Objective To study the chemical constituents of Anthogorgia sp. collected from Beihai area of Guangxi Zhuang Autonomous Region, China. Methods The Et_2 O extract of Anthogorgia sp. was subjected to repeated silica gel (eluted with Pe: Ac=99 : 1, 49: 1, 39: 1, 34 : 1, 29: 1, 24 : 1, 19: 1, 14 : 1, 11 : 1, 9 : 1, 8: 1, 7 : 1, 6 : 1, 5 : 1,4: 1, 3: 1, 2: 1, 1 : 1, 100%Ac; CHCl_3 : MeOH=24: 1, 19: 1, 14 : 1, 9: 1, 7: 1, 5: 1, 3 : 1, 1 : 1, 1 : 4, 1 : 9,1 : 39, 100% MeOH) and Sephadex LH-20 column chromatography(n-hexane : CHCl_3 : MeOH=2 : 1 : 1) for isolation and purification. The structures of the resultant compounds were elucidated using spectroscopic analysis(~1H NMR, ~(13)C NMR and MS). Results Six steroids and one ceramide were isolated and identified as: Ergost-5,24(28)-dien-3β-ol(1), (22E,24R)-ergost-7,22-dien-3β, 5α, 6β-triol (2), (22 E, 24 S)-ergost-5,22-dien-3β-ol (3), cholesta-5-en-3β, 7β, 19α-triol (4), (22E)-cholesta-5,22-dien-3β-ol(5), cholesterol(6), and N-palmitoyloctadecasphinga-4(E)-ene(7). Conclusion All the six compounds are isolated from Anthogorgia sp. for the first time.