抗核抗体谱检测在自身免疫性疾病诊断中的价值

目的：分析自身免疫性疾病（autoimmune disease， AID）患者抗核抗体（antinuclear anti-body， ANA）谱检测结果，评价ANA谱在诊断AID中的应用价值。方法选择2012年5月-2013年5月于我院就诊的AID患者513例，非AID患者353例，并对其ANA谱的检测结果进行回顾性分析。结果系统性红斑狼疮组抗dsDNA抗体、抗组蛋白抗体、抗核小体抗体、抗SM抗体、抗SSA抗体、抗rRNP抗体阳性率均高于对照组，且差异均有统计学意义（P均〈0．05）；混合性结缔组织病组抗U1RNP抗体、抗SSA抗体阳性率均高于对照组，且差异均有统计学意义（P均〈0．05）；干燥综合征组抗SSA抗体、抗SSB抗体阳性率均高于对照组，且差异均有统计学意义（P均〈0．05）；进行性系统性硬化病组抗SCL-70抗体、抗CENP-B抗体阳性率均高于对照组，且差异均有统计学意义（P均〈0．05）；多发性肌炎/皮肌炎组抗JO-1抗体阳性率高于对照组，且差异有统计学意义（P〈0．05）。当抗组蛋白抗体、抗核小体抗体、抗rRNP抗体和抗dsDNA抗体同时阳性时，系统性红斑狼疮组阳性率显著高于混合性结缔组织病组，且差异有统计学意义（P〈0．05）。结论 ANA谱检测对AID的诊断及鉴别诊断有重要价值。

The value of antinuclear antibody series in autoimmune diseases diagnosis

Objective To evaluate the value of antinuclear antibody（ANA） series tests in the diagno-sis of autoimmune diseases（AID）. Methods 513 cases AID patients and 353 cases without AID patients were selected from May 2012 to May 2013. And results of ANA series tests in these patients were analyzed retro-spectively. Results The positive rates of anti-dsDNA antibody, anti-histone antibody, anti-nucleosomes an-tibody, anti-SM antibody, anti-SSA antibody, anti-rRNP antibody in systemic lupus erythematosus （SLE） group were all higher than those of control group , and the differences all had statistical significance （Pall〈0.05）. The positive rates of anti-U1RNP antibody and anti-SSA antibody in mixed connective tissue disease （MCTD） were all higher than those of control group, and the differences all had statistical significance （Pall〈0.05）. The positive rates of anti-SSA antibody and anti-SSB antibody in primary Sjogren＆#39;s syndrome group, and anti-SCL-70 antibody and anti-CENP-B antibody in progressive systemic sclerosis group , and anti-JO-1 antibody in polymyositis and dermatomyositis group were all higher than those of control group , and the differ-ences all had statistical significance（Pall〈0.05）. The positive rate of anti-histone antibody, anti-nucleosomes antibody, anti-rRNP antibody and anti-dsDNA antibody all together positive in SLE group was higher than that of MCTD group, and the difference had statistical significance （P〈0.05）. Conclusion ANA series tests have great significance in the discrimination diagnosis and diagnosis of the AID.