血管紧张素Ⅱ诱导内皮细胞产生IL-8和Duffy抗原/趋化因子受体的研究

目的:探讨Duffy抗原/趋化因子受体(DARC)和IL-8在血管紧张素II(AngⅡ)致炎机制中可能的作用.方法:体外培养人脐静脉内皮细胞,分别用PBS(对照组)、AngⅡ(10-7 mol/L)诱导0～24 h,收集0、6、12、24 h 4个时间点的细胞培养上清和内皮细胞,采用ELISA法分别检测细胞培养上清和内...

Angiotensin II stimulates production of interleukin-8 and Duffy antigen/receptor for chemokine from endothelial cells

AIM: To explore the roles of interleukin-8 (IL-8) and Duffy antigen/receptor for chemokine (DARC) in AngII-induced inflammation in endothelial cells. METHODS: Endothelial cells from human umbilical vein (HUVEC) were cultured and stimulated with Ang II (10-7 mol/L) for 0-24 h with phosphate-buffered saline (PBS）as the control. The supernatant and cell lyses of endothelial cells were collected at 0, 6, 12 and 24 h. IL-8 levels were measured by enzyme-linked immunosorbent assay (ELISA). DARC mRNA expression in endothelial cells was measured by real-time polymerase chain reaction (real-time PCR). RESULTS: AngII（10-7mol/L）stimulated IL-8 production in a time-dependent manner. IL-8 level in the supernatant increased gradually during 0-6 h and decreased at 24 h. IL-8 level in cell lyses also increased during 6-24 h in a time-dependent manner, but the changes were not parallel with those in the supernatant. Ang II also stimulated DARC mRNA expression during 6-24 h in a time-dependent manner. CONCLUSION: AngII stimulates IL-8 release and DARC expression from HUVECs. IL-8 and DARC may be involved in the process of AngII-induced inflammation, suggesting that DARC binds IL-8 to form chemokine concentration gradient in inflammation.